LOCUS       sequence-85778-e        1213 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: mCerulean-H2B-6
FEATURES             Location/Qualifiers
     source          1..1213
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..432
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      445..454
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(451..453,454..456,457..1170)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(496..517)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(757..776)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1104..1125
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggactcagat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc caccatgcca
       61 gagccagcga agtctgctcc cgccccgaaa aagggctcca agaaggcggt gactaaggcg
      121 cagaagaaag gcggcaagaa gcgcaagcgc agccgcaagg agagctattc catctatgtg
      181 tacaaggttc tgaagcaggt ccaccctgac accggcattt cgtccaaggc catgggcatc
      241 atgaattcgt ttgtgaacga cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg
      301 cattacaaca agcgctcgac catcacctcc agggagatcc agacggccgt gcgcctgctg
      361 ctgcctgggg agttggccaa gcacgccgtg tccgagggta ctaaggccat caccaagtac
      421 accagcgcta aggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      481 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      541 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      601 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag
      661 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      721 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      781 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      841 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacgccat cagcgacaac
      901 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
      961 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1021 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
     1081 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1141 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc
     1201 ataccacatt gag
//
