LOCUS       sequence-85799-e        2244 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 433, Emission: 
            475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2244)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2244
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1473..1482
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1479..1481,1482..1484,1485..2198)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1524..1545)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1785..1804)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2132..2153
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaataccca
       61 tgtccaccag gtccgtgtcc tcgtcctcct accgcaggat gttcggcggc ccgggcaccg
      121 cgagccggcc gagctccagc cggagctacg tgactacgtc cacccgcacc tacagcctgg
      181 gcagcgcgct gcgccccagc accagccgca gcctctacgc ctcgtccccg ggcggcgtgt
      241 atgccacgcg ctcctctgcc gtgcgcctgc ggagcagcgt gcccggggtg cggctcctgc
      301 aggactcggt ggacttctcg ctggccgacg ccatcaacac cgagttcaag aacacccgca
      361 ccaacgagaa ggtggagctg caggagctga atgaccgctt cgccaactac atcgacaagg
      421 tgcgcttcct ggagcagcag aataagatcc tgctggccga gctcgagcag ctcaagggcc
      481 aaggcaagtc gcgcctgggg gacctctacg aggaggagat gcgggagctg cgccggcagg
      541 tggaccagct aaccaacgac aaagcccgcg tcgaggtgga gcgcgacaac ctggccgagg
      601 acatcatgcg cctccgggag aaattgcagg aggagatgct tcagagagag gaagccgaaa
      661 acaccctgca atctttcaga caggatgttg acaatgcgtc tctggcacgt cttgaccttg
      721 aacgcaaagt ggaatctttg caagaagaga ttgccttttt gaagaaactc cacgaagagg
      781 aaatccagga gctgcaggct cagattcagg aacagcatgt ccaaatcgat gtggatgttt
      841 ccaagcctga cctcacggct gccctgcgtg acgtacgtca gcaatatgaa agtgtggctg
      901 ccaagaacct gcaggaggca gaagaatggt acaaatccaa gtttgctgac ctctctgagg
      961 ctgccaaccg gaacaatgac gccctgcgcc aggcaaagca ggagtccact gagtaccgga
     1021 gacaggtgca gtccctcacc tgtgaagtgg atgcccttaa aggaaccaat gagtccctgg
     1081 aacgccagat gcgtgaaatg gaagagaact ttgccgttga agctgctaac taccaagaca
     1141 ctattggccg cctgcaggat gagattcaga atatgaagga ggaaatggct cgtcaccttc
     1201 gtgaatacca agacctgctc aatgttaaga tggcccttga cattgagatt gccacctaca
     1261 ggaagctgct ggaaggcgag gagagcagga tttctctgcc tcttccaaac ttttcctccc
     1321 tgaacctgag ggaaactaat ctggattcac tccctctggt tgatacccac tcaaaaagga
     1381 cacttctgat taagacggtt gaaactagag atggacaggt tatcaacgaa acttctcagc
     1441 atcacgatga ccttgaaggg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc
     1501 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1561 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1621 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
     1681 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1741 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1801 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1861 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacgccatca
     1921 gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     1981 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     2041 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc
     2101 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2161 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat
     2221 aatcagccat accacattgt agag
//
