LOCUS       sequence-85810-e        1392 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..1392
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(33..35,36..38,39..749)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgagc tggggcgtgc
      241 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacgcc atccacggca
      481 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc ggcctcaact
      541 gcaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      721 tcactctcgg catggacgag ctgtacaagt ccggactcag atctggcagc agtggaggtt
      781 ctgggggcaa atacgtagac tcggagggac atctctacac cgttcccatc cgggaacagg
      841 gcaacatcta caagcccaac aacaaggcca tggcagacga gctgagcgag aagcaagtgt
      901 acgacgcgca caccaaggag atcgacctgg tcaaccgcga ccctaaacac ctcaacgatg
      961 acgtggtcaa gattgacttt gaagatgtga ttgcagaacc agaagggaca cacagttttg
     1021 acggcatttg gaaggccagc ttcaccacct tcactgtgac gaaatactgg ttttaccgct
     1081 tgctgtctgc cctctttggc atcccgatgg cactcatctg gggcatttac ttcgccattc
     1141 tctctttcct gcacatctgg gcagttgtac catgcattaa gagcttcctg attgagattc
     1201 agtgcatcag ccgtgtctat tccatctacg tccacaccgt ctgtgaccca ctctttgaag
     1261 ctgttgggaa aatattcagc aatgtccgca tcaacttgca gaaagaaata taagaattct
     1321 gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc
     1381 cataccacat tt
//
