LOCUS       sequence-85829-e        2123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Keratin-17
FEATURES             Location/Qualifiers
     source          1..2123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1358..1367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1364..1366,1367..1369,1370..2083)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1409..1430)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1670..1689)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2017..2038
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagca ccatgagctt caccactcgc tccaccttct ccaccaacta
       61 ccggtccctg ggctctgtcc aggcgcccag ctacggcgcc cggccggtca gcagcgcggc
      121 cagcgtctat gcaggcgctg ggggctctgg ttcccggatc tccgtgtccc gctccaccag
      181 cttcaggggc ggcatggggt ccgggggcct ggccaccggg atagccgggg gtctggcagg
      241 aatgggaggc atccagaacg agaaggagac catgcaaagc ctgaacgacc gcctggcctc
      301 ttacctggac agagtgagga gcctggagac cgagaaccgg aggctggaga gcaaaatccg
      361 ggagcacttg gagaagaagg gaccccaggt cagagactgg agccattact tcaagatcat
      421 cgaggacctg agggctcaga tcttcgcaaa tactgtggac aatgcccgca tcgttctgca
      481 gattgacaat gcccgtcttg ctgctgatga ctttagagtc aagtatgaga cagagctggc
      541 catgcgccag tctgtggaga acgacatcca tgggctccgc aaggtcattg atgacaccaa
      601 tatcacacga ctgcagctgg agacagagat cgaggctctc aaggaggagc tgctcttcat
      661 gaagaagaac cacgaagagg aagtaaaagg cctacaagcc cagattgcca gctctgggtt
      721 gaccgtggag gtagatgccc ccaaatctca ggacctcgcc aagatcatgg cagacatccg
      781 ggcccaatat gacgagctgg ctcggaagaa ccgagaggag ctagacaagt actggtctca
      841 gcagattgag gagagcacca cagtgctcac cacacagtct gctgaggttg gagctgctga
      901 gacgacgctc acagagctga gacgtacagt ccagtccttg gagatcgacc tggactccat
      961 gagaaatctg aaggccagct tggagaacag cctgagggag gtggaggccc gctacgccct
     1021 acagatggag cagctcaacg ggatcctgct gcaccttgag tcagagctgg cacagacccg
     1081 ggcagaggga cagcgccagg cccaggagta tgaggccctg ctgaacatca aggtcaagct
     1141 ggaggctgag atcgccacct accgccgcct gctggaagat ggcgaggact ttaatcttgg
     1201 tgatgccttg gacagcagca actccatgca aaccatccaa aagaccacca cccgccggat
     1261 agtggatggc aaagtggtgt ctgagaccaa tgacaccaaa gttctgaggc atcgaattct
     1321 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga
     1381 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1441 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1501 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgag
     1561 ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1621 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1681 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1741 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc
     1801 catccacggc aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     1861 cggcctcaac tgcaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1921 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1981 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2041 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag
     2101 atcataatca gccataccac att
//
