LOCUS       sequence-85831-e        1599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Lasp1-N-10
FEATURES             Location/Qualifiers
     source          1..1599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      214..223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(840..842,843..845,846..1559)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(885..906)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1146..1165)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1493..1514
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaacggtcag atccgctagc gccaccatga accccaactg cgcccggtgc ggcaagatcg
       61 tgtatcccac ggagaaggtg aactgtctgg ataagttctg gcataaagca tgcttccatt
      121 gcgagacctg caagatgaca ctgaacatga agaactacaa gggctacgag aagaagccct
      181 actgcaacgc acactacccc aagcagtcct tcaccatggt ggcggacacc ccggaaaacc
      241 ttcgcctcaa gcaacagagt gagctccaga gtcaggtgcg ctacaaggag gagtttgaga
      301 agaacaaggg caaaggtttc agcgtagtgg cagacacgcc cgagctccag agaatcaaga
      361 agacccagga ccagatcagt aacataaaat accatgagga gtttgagaag agccgcatgg
      421 gccctagcgg gggcgagggc atggagccag agcgtcggga ttcacaggac ggcagcagct
      481 accggcggcc cctggagcag cagcagcctc accacatccc gaccagtgcc ccggtttacc
      541 agcagcccca gcagcagccg gtggcccagt cctatggtgg ctacaaggag cctgcagccc
      601 cagtctccat acagcgcagc gccccaggtg gtggcgggaa gcggtaccgc gtggtgtatg
      661 actacagcgc cgccgacgag gacgaggtct ccttccagga cggggacacc atcgtcaacg
      721 tgcagcagat cgacgacggc tggatgtacg ggacggtgga gcgcaccggc gacacgggga
      781 tgctgccggc caactacgtg gaggccatca gctccggagg ggatccaccg gtcgccacca
      841 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      901 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      961 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1021 tcgtgaccac cctgagctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
     1081 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1141 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1201 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1261 agctggagta caacgccatc cacggcaacg tctatatcac cgccgacaag cagaagaacg
     1321 gcatcaaggc caacttcggc ctcaactgca acatcgagga cggcagcgtg cagctcgccg
     1381 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1441 acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1501 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa
     1561 gcggccgcga ctctagatca taatcagcca taccacatt
//
