LOCUS       sequence-85837-e        1872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-MCHR1-N-10
FEATURES             Location/Qualifiers
     source          1..1872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1108..1117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1114..1116,1117..1119,1120..1833)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1159..1180)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1420..1439)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1767..1788
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatggat ctgcaagcct cgttgctgtc cactggcccc
       61 aatgccagca acatctccga tggccaggat aatttcacat tggcggggcc acctcctcgc
      121 acaaggagtg tctcctacat caacatcatc atgccttcag tgtttggtac catctgtctc
      181 ctgggcattg tgggaaactc cacagtcatt tttgccgtgg tgaagaaatc caagctgcac
      241 tggtgcagca acgtccctga catcttcatc atcaacctct ctgtggtgga tctgcttttc
      301 ctgctgggca tgcctttcat gatccaccag ctcatgggta atggtgtctg gcactttggg
      361 gaaaccatgt gcaccctcat cacagccatg gacgccaaca gtcagttcac cagcacctac
      421 atcctgactg ctatggccat tgaccgctac ttggccaccg tccatcccat ctcctccacc
      481 aagttccgga agccctccat ggccaccctg gtgatctgcc tcctgtgggc tctctcgttc
      541 attagcatca ctcctgtgtg gctctatgcc aggcttatcc ccttcccagg gggtgctgtg
      601 ggctgtggca tccgcctacc aaacccagat actgatcttt actggttcac tctgtatcag
      661 tttttcctgg ccttcgccct tccgtttgtg gtcatcactg ctgcgtacgt gaaaatacta
      721 cagcgcatga cgtcttcggt ggccccagcc tctcaacgca gcatccggct tcggacaaag
      781 agggtgaccc gcacagccat tgccatctgt ctggtcttct ttgtgtgctg ggcgccctac
      841 tacgtgctgc agctgaccca gttgtccatc agccgcccga ccctcacatt cgtctacctg
      901 tacaatgcgg ctatcagctt gggctatgcc aacagctgcc tcaatccctt tgtgtacata
      961 gtactctgtg agacctttcg aaaacgcttg gtgctgtcgg tgaagcccgc ggcccagggg
     1021 cagcttcgca cggtcagcaa tgctcagaca gctgacgagg agaggacaga aagcaaaggc
     1081 accggaggta gcggggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
     1141 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1201 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1261 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgag ctggggcgtg
     1321 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1381 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1441 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1501 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc catccacggc
     1561 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt cggcctcaac
     1621 tgcaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1681 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
     1741 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1801 atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca
     1861 gccataccac at
//
