LOCUS       sequence-85843-e        2715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Fimbrin/Cytoskeleton, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Plastin-C-10
FEATURES             Location/Qualifiers
     source          1..2715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             2112..2120
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgag ctggggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc catccacggc
      481 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt cggcctcaac
      541 tgcaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggaagcg gctccgggag cggaagcggg
      781 gaaaacagta ctactaccat ttctcgggag gagcttgaag aactacaaga ggcatttaat
      841 aaaatagata ttgacaatag tgggtatgtc agtgactatg aacttcaaga cctgtttaag
      901 gaagcaagcc ttcctctgcc tggctacaag gtgcgcgaga ttgtggagaa aattctatca
      961 gttgctgaca gcaacaaaga tggcaaaatc agttttgaag agtttgtgtc actaatgcaa
     1021 gaattaaaaa gcaaagatat cagcaaaaca ttccgaaaaa taattaacaa gagggaaggg
     1081 attactgcta ttggaggaac ttcaactatt tccagtgagg gcacacagca ttcttattca
     1141 gaggaagaaa aagtggcttt tgttaactgg ataaacaaag ccctggagaa tgaccctgac
     1201 tgtaagcatc ttatacccat gaatcccaat gatgatagtc ttttcaagtc acttgcagat
     1261 ggcatccttc tttgcaaaat gatcaactta tctgaaccag atacaattga tgaaagagcc
     1321 atcaataaga aaaagctcac gccattcact atttctgaaa atttaaacct agctctgaat
     1381 tctgcctcag ccattggttg tacagtggtc aacattggtg catcagatct caaagaagga
     1441 aaacctcact tggtcttggg acttctctgg cagatcatca aagttggcct ttttgctgat
     1501 attgagattt ccaggaatga agctctgatt gcattgttaa atgaaggtga ggaactagag
     1561 gagctgatga agctttctcc cgaggaatta ctgctgcgat gggtgaacta ccatctgacc
     1621 aatgcaggat ggcataccat cagcaacttc agccaagaca ttaaggactc gagagcctat
     1681 tttcatctgc ttaatcagat tgcccctaaa ggtggggaag atggacctgc cattgccatt
     1741 gacctttcag gaattaatga gacaaatgac ctgaagcgtg ctggactcat gcttcaagaa
     1801 gcagataaac tgggctgcaa acagtttgtt actcctgcag atgtggtttc aggcaatcct
     1861 aaacttaatt tagcttttgt agctaatttg tttaacacat acccgtgcct gcacaagccg
     1921 aataataatg acatcgatat gaatttactg gaaggagaga gcaaggaaga gagaacattt
     1981 cggaactgga tgaattcctt gggagtcaac ccatacatta atcatttgta cagtgacctt
     2041 gcagatgctt tagtgatctt tcagctctat gagatgatcc gagtgccagt caactggagc
     2101 catgtcaaca aacctcctta tcctgccctt ggagggaaca tgaagaagat tgaaaactgt
     2161 aactatgcag tggaacttgg gaagaacaag gccaaattct ccttggttgg cattgctggg
     2221 caggacctaa atgaagggaa ttcaacactt accctggcat tggtatggca gctgatgaga
     2281 aggtacacat tgaatgtgtt atcggatctt ggagagggtg aaaaagtaaa tgatgaaatt
     2341 ataattaaat gggtcaatca gactcttaaa agtgcaaaca aaaagacttc tatttccagc
     2401 ttcaaggata aatctataag cacaagttta cctgtcctag atttaataga tgccattgca
     2461 ccaaatgcag ttcgtcaaga aatgatcagg agagaaaact tatctgatga ggacaagctg
     2521 aacaatgcta aatacgccat ttcagttgct cgaaagatcg gtgcccggat atatgcatta
     2581 cctgatgacc tcgtagaagt gaaaccaaag atggttatga cggtgtttgc atgcttaatg
     2641 ggaaaaggac tgaacagaat aaaataagga tccaccggat ctagataact gatcataatc
     2701 agccatacca cattt
//
