LOCUS       sequence-85855-e        2191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg agctggggcg
      241 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac gccatccacg
      481 gcaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcggcctca
      541 actgcaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggact cagatctggc agcggtggag
      781 gcagcgcatc cggcggaagc ggaagcgtgc gtgagtgcat ctccatccac gttggccagg
      841 ctggtgtcca gattggcaat gcctgctggg agctctactg cctggaacac ggcatccagc
      901 ccgatggcca gatgccaagt gacaagacca ttgggggagg agatgactcc ttcaacacct
      961 tcttcagtga gacgggcgct ggcaagcacg tgccccgggc tgtgtttgta gacttggaac
     1021 ccacagtcat tgatgaagtt cgcactggca cctaccgcca gctcttccac cctgagcagc
     1081 tcatcacagg caaggaagat gctgccaata actatgcccg agggcactac accattggca
     1141 aggagatcat tgaccttgtg ttggaccgaa ttcgcaagct ggctgaccag tgcaccggtc
     1201 ttcagggctt cttggttttc cacagctttg gtgggggaac tggttctggg ttcacctccc
     1261 tgctcatgga acgtctctca gttgattatg gcaagaagtc caagctggag ttctccattt
     1321 acccagcacc ccaggtttcc acagctgtag ttgagcccta caactccatc ctcaccaccc
     1381 acaccaccct ggagcactct gattgtgcct tcatggtaga caatgaggcc atctatgaca
     1441 tctgtcgtag aaacctcgat atcgagcgcc caacctacac taaccttaac cgccttatta
     1501 gccagattgt gtcctccatc actgcttccc tgagatttga tggagccctg aatgttgacc
     1561 tgacagaatt ccagaccaac ctggtgccct acccccgcat ccacttccct ctggccacat
     1621 atgcccctgt catctctgct gagaaagcct accatgaaca gctttctgta gcagagatca
     1681 ccaatgcttg ctttgagcca gccaaccaga tggtgaaatg tgaccctcgc catggtaaat
     1741 acatggcttg ctgcctgttg taccgtggtg acgtggttcc caaagatgtc aatgctgcca
     1801 ttgccaccat caaaaccaag cgcagcatcc agtttgtgga ttggtgcccc actggcttca
     1861 aggttggcat caactaccag cctcccactg tggtgcctgg tggagacctg gccaaggtac
     1921 agagagctgt gtgcatgctg agcaacacca cagccattgc tgaggcctgg gctcgcctgg
     1981 accacaagtt tgacctgatg tatgccaagc gtgcctttgt tcactggtac gtgggtgagg
     2041 ggatggagga aggcgagttt tcagaggccc gtgaagatat ggctgccctt gagaaggatt
     2101 atgaggaggt tggtgtggat tctgttgaag gagagggtga ggaagaagga gaggaatact
     2161 aaggatccac cggatctaga taactgatca t
//
