LOCUS       sequence-85892-e        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: GalT Targeting Sequence, Excitation: 462, Emission: 
            492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      314..323
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             320..1030
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 ggggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcatgaggct
       61 tcgggagccg ctcctgagcg gcagcgccgc gatgccaggc gcgtccctac agcgggcctg
      121 ccgcctgctc gtggccgtct gcgctctgca ccttggcgtc accctcgttt actacctggc
      181 tggccgcgac ctgagccgcc tgccccaact ggtcggagtc tccacaccgc tgcagggcgg
      241 ctcgaacagt gccgccgcca tcgggcagtc ctccggggag ctccggaccg gaggggccaa
      301 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag accacaatgg gcgtaatcaa
      361 gcccgacatg aagatcaagc tgaagatgga gggcaacgtg aatggccacg ccttcgtgat
      421 cgagggcgag ggcgagggca agccctacga cggcaccaac accatcaacc tggaggtgaa
      481 ggagggagcc cccctgccct tctcctacga cattctgacc accgcgttcg cctacggcaa
      541 cagggccttc accaagtacc ccgacgacat ccccaactac ttcaagcagt ccttccccga
      601 gggctactct tgggagcgca ccatgacctt cgaggacaag ggcatcgtga aggtgaagtc
      661 cgacatctcc atggaggagg actccttcat ctacgagata cacctcaagg gcgagaactt
      721 cccccccaac ggccccgtga tgcagaagaa gaccaccggc tgggacgcct ccaccgagag
      781 gatgtacgtg cgcgacggcg tgctgaaggg cgacgtcaag cacaagctgc tgctggaggg
      841 cggcggccac caccgcgttg acttcaagac catctacagg gccaagaagg cggtgaagct
      901 gcccgactat cactttgtgg accaccgcat cgagatcctg aaccacgaca aggactacaa
      961 caaggtgacc gtttacgaga gcgccgtggc ccgcaactcc accgacggca tggacgagct
     1021 gtacaagtaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
     1081 tacttgcttt aaaaa
//
