LOCUS       sequence-85914-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      218..227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             224..934
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcgcc accatgattc acaccaacct
       61 gaagaaaaag ttcagctgct gcgtcctggt ctttcttctg tttgcagtca tctgtgtgtg
      121 gaaggaaaag aagaaaggga gttactatga ttcctttaaa ttgcaaacca aggaattcct
      181 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga
      241 ggagaccaca atgggcgtaa tcaagcccga catgaagatc aagctgaaga tggagggcaa
      301 cgtgaatggc cacgccttcg tgatcgaggg cgagggcgag ggcaagccct acgacggcac
      361 caacaccatc aacctggagg tgaaggaggg agcccccctg cccttctcct acgacattct
      421 gaccaccgcg ttcgcctacg gcaacagggc cttcaccaag taccccgacg acatccccaa
      481 ctacttcaag cagtccttcc ccgagggcta ctcttgggag cgcaccatga ccttcgagga
      541 caagggcatc gtgaaggtga agtccgacat ctccatggag gaggactcct tcatctacga
      601 gatacacctc aagggcgaga acttcccccc caacggcccc gtgatgcaga agaagaccac
      661 cggctgggac gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga agggcgacgt
      721 caagcacaag ctgctgctgg agggcggcgg ccaccaccgc gttgacttca agaccatcta
      781 cagggccaag aaggcggtga agctgcccga ctatcacttt gtggaccacc gcatcgagat
      841 cctgaaccac gacaaggact acaacaaggt gaccgtttac gagagcgccg tggcccgcaa
      901 ctccaccgac ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca
      961 gccataccac att
//
