LOCUS       sequence-85958-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: CAAX - Membrane, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(32..34,35..37,38..748)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(338..357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     685..706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
       61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca
      241 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagcgacaa
      481 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      541 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
      661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      721 cactctcggc atggacgagc tgtacaagtc cggactcaga tctaagctga accctcctga
      781 tgagagtggc cccggctgca tgagctgcaa gtgtgtgctc tcctgagaat tctgcagtcg
      841 acggtaccgc gggcccg
//
