LOCUS       sequence-85967-e        2479 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2479)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Hc-SRC-N-18
FEATURES             Location/Qualifiers
     source          1..2479
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1706..1715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1712..1714,1715..1717,1718..2431)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1757..1778)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2018..2037)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2365..2386
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 taacggtcgg atgccgctag cgctaccgga ctcagatctc gagaccgcca tgggtagcaa
       61 caagagcaag cccaaggatg ccagccagcg gcgccgcagc ctggagcccg ccgagaacgt
      121 gcacggcgct ggcgggggcg ctttccccgc ctcgcagacc cccagcaagc cagcctcggc
      181 cgacggccac cgcggcccca gcgcggcctt cgcccccgcg gccgccgagc ccaagctgtt
      241 cggaggcttc aactcctcgg acaccgtcac ctccccgcag agggcgggcc cgctggccgg
      301 tggagtgacc acctttgtgg ccctctatga ctatgagtct aggacggaga cagacctgtc
      361 cttcaagaaa ggcgagcggc tccagattgt caacaacaca gagggagact ggtggctggc
      421 ccactcgctc agcacaggac agacaggcta catccccagc aactacgtgg cgccctccga
      481 ctccatccag gctgaggagt ggtattttgg caagatcacc agacgggagt cagagcggtt
      541 actgctcaat gcagagaacc cgagagggac cttcctcgtg cgagaaagtg agaccacgaa
      601 aggtgcctac tgcctctcag tgtctgactt cgacaacgcc aagggcctca acgtgaagca
      661 ctacaagatc cgcaagctgg acagcggcgg cttctacatc acctcccgca cccagttcaa
      721 cagcctgcag cagctggtgg cctactactc caaacacgcc gatggcctgt gccaccgcct
      781 caccaccgtg tgccccacgt ccaagccgca gactcagggc ctggccaagg atgcctggga
      841 gatccctcgg gagtcgctgc ggctggaggt caagctgggc cagggctgct ttggcgaggt
      901 gtggatgggg acctggaacg gtaccaccag ggtggccatc aaaaccctga agcctggcac
      961 gatgtctcca gaggccttcc tgcaggaggc ccaggtcatg aagaagctga ggcatgagaa
     1021 gctggtgcag ttgtatgctg tggtttcaga ggagcccatt tacatcgtca cggagtacat
     1081 gagcaagggg agtttgctgg actttctcaa gggggagaca ggcaagtacc tgcggctgcc
     1141 tcagctggtg gacatggctg ctcagatcgc ctcaggcatg gcgtacgtgg agcggatgaa
     1201 ctacgtccac cgggaccttc gtgcagccaa catcctggtg ggagagaacc tggtgtgcaa
     1261 agtggccgac tttgggctgg ctcggctcat tgaagacaat gagtacacgg cgcggcaagg
     1321 tgccaaattc cccatcaagt ggacggctcc agaagctgcc ctctatggcc gcttcaccat
     1381 caagtcggac gtgtggtcct tcgggatcct gctgactgag ctcaccacaa agggacgggt
     1441 gccctaccct gggatggtga accgcgaggt gctggaccag gtggagcggg gctaccggat
     1501 gccctgcccg ccggagtgtc ccgagtccct gcacgacctc atgtgccagt gctggcggaa
     1561 ggagcctgag gagcggccca ccttcgagta cctgcaggcc ttcctggagg actacttcac
     1621 gtccaccgag ccccagtacc agcccgggga gaacctcggg cggagtaggg gtcgcagagg
     1681 cagcaagcgg aagggtggac cggtcgccac catggtgagc aagggcgagg agctgttcac
     1741 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1801 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1861 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca
     1921 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1981 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2041 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2101 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagcgacaa
     2161 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     2221 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2281 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     2341 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2401 cactctcggc atggacgagc tgtacaagta aagcggccgc gacttctaga tcataatcag
     2461 ccataccaca tttgtagag
//
