LOCUS       sequence-85988-e        1900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1900)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Sequestosome1-N-18
FEATURES             Location/Qualifiers
     source          1..1900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1137..1146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1143..1145,1146..1148,1149..1862)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1188..1209)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1449..1468)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1796..1817
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatccgc tagcgccacc atggccatgt cctacgtgaa ggatgacatc ttccgaatct
       61 acattaaaga gaaaaaagag tgccggcggg accaccgccc accgtgtgct caggaggcgc
      121 cccgcaacat ggtgcacccc aatgtgatct gcgatggctg caatgggcct gtggtaggaa
      181 cccgctacaa gtgcagcgtc tgcccagact acgacttgtg tagcgtctgc gagggaaagg
      241 gcttgcaccg ggggcacacc aagctcgcat tccccagccc cttcgggcac ctgtctgagg
      301 gcttctcgca cagccgctgg ctccggaagg tgaaacacgg acacttcggg tggccaggat
      361 gggaaatggg tccaccagga aactggagcc cacgtcctcc tcgtgcaggg gaggcccgcc
      421 ctggccccac ggcagaatca gcttctggtc catcggagga tccgagtgtg aatttcctga
      481 agaacgttgg ggagagtgtg gcagctgccc ttagccctct gggcattgaa gttgatatcg
      541 atgtggagca cggagggaaa agaagccgcc tgacccccgt ctctccagag agttccagca
      601 cagaggagaa gagcagctca cagccaagca gctgctgctc tgatcccagc aagccgggtg
      661 ggaatgttga gggcgccacg cagtctctgg cggagcagat gagaaagatc gccttggagt
      721 ccgaggggcg ccctgaggaa cagatggagt cggataactg ttcaggagga gatgatgact
      781 ggacccatct gtcttcaaaa gaagtggacc cgtctacagg tgaactccag tccctacaga
      841 tgccagaatc cgaagggcca agctctctgg acccctccca ggagggaccc acagggctga
      901 aggaagctgc cttgtaccca catctcccgc cagaggctga cccgcggctg attgagtccc
      961 tctcccagat gctgtccatg ggcttctctg atgaaggcgg ctggctcacc aggctcctgc
     1021 agaccaagaa ctatgacatc ggagcggctc tggacaccat ccagtattca aagcatcccc
     1081 cgccgttgag cagcggtgga gcaagcgcag ccagtggtag cgcagcggga ccggtcgcca
     1141 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1201 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     1261 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     1321 ccctcgtgac caccctgtcc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga
     1381 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     1441 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1501 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1561 acaagctgga gtacaactac atcagcgaca acgtctatat caccgccgac aagcagaaga
     1621 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
     1681 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1741 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1801 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     1861 aaagcggccg cgactctaga tcataatcag ccataccaca
//
