LOCUS       sequence-85990-e        6024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6024)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Tensin1-C-14
FEATURES             Location/Qualifiers
     source          1..6024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(21..23,24..26,27..737)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(66..87)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(327..346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     674..695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
       61 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      121 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      181 tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc
      241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      421 acggcaacat cctggggcac aagctggagt acaactacat cagcgacaac gtctatatca
      481 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      541 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      601 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
      661 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      721 tggacgagct gtacaagtcc ggaagtgctg ggagcgcaag cggaagcgga ggctccggta
      781 gtgtgagccg gaccatggag gacagctgtg agctggacct ggtgtacgtc acagagagga
      841 tcatcgctgt ctccttcccc agcacagcca atgaggagaa ctttcggagc aacctccgtg
      901 aggtggcgca gatgctcaag tccaaacatg gaggcaacta cctgctgttc aacctctctg
      961 agcggagacc tgacatcacg aagctccatg ccaaggtact ggaatttggc tggcccgacc
     1021 tccacacccc agccctggag aagatatgca gcatctgtaa ggccatggac acatggctca
     1081 atgcagaccc tcacaatgtc gttgttctac acaacaaggg aaaccgaggc aggataggag
     1141 ttgtcatcgc ggcttacatg cactacagca acatttctgc cagtgcggac caggctctgg
     1201 atcggtttgc aatgaagcgg ttctatgagg ataagattgt gcccattggc cagccatccc
     1261 aaagaaggta cgtgcattac ttcagtggcc tgctctccgg ctccatcaaa atgaacaaca
     1321 agcccttgtt tctgcaccac gtgatcatgc acggcatccc caactttgag tctaaaggag
     1381 gatgtcggcc atttctccgc atctaccagg ccatgcaacc tgtgtacaca tctggcatct
     1441 acaacatccc aggagacagc cagactagcg tctgcatcac catcgagcca ggactgctct
     1501 tgaagggaga catcttgctg aagtgctacc acaagaagtt ccgaagccca gcccgagacg
     1561 tcatcttccg tgtgcagttc cacacctgtg ccatccatga cctgggggtt gtctttggga
     1621 aggaggacct tgatgatgct ttcaaagatg atcgatttcc agagtatggc aaagtggagt
     1681 ttgtattttc ttatgggcca gagaaaattc aaggcatgga gcacctggag aacgggccga
     1741 gcgtgtctgt ggactataac acctccgacc ccctcatccg ctgggactcc tacgacaact
     1801 tcagtgggca tcgagatgac ggcatggagg aggtggtggg acacacgcag gggccactag
     1861 atgggagcct gtatgctaag gtgaagaaga aagactccct gcacggcagc accggggctg
     1921 ttaatgccac acgtcctaca ctgtcggcca cccccaacca cgtggaacac acgctttctg
     1981 tgagcagcga ctcgggcaac tccacagcct ccaccaagac cgacaagacc gacgagcctg
     2041 tccccggggc ctccagtgcc actgctgcct tgagtcccca ggagaagcgg gagctggacc
     2101 gcctgctgag tggctttggc ttagagcgag agaagcaagg cgccatgtac cacacccagc
     2161 acctcaggtc ccgcccagca gggggctcgg ctgtgccctc ctctggacgc cacgttgtcc
     2221 cagcccaggt tcatgtcaat ggtggggcgt tagcatctga gcgggagaca gacatcctgg
     2281 acgatgaatt gccaaaccag gatggtcaca gtgcgggcag catgggcaca ctctcttctc
     2341 tggacggggt caccaacacc agtgaggggg gctacccaga ggccctgtcc ccactgacca
     2401 acggtctgga caagtcctac cccatggagc ctatggtcaa tggaggaggc tacccctacg
     2461 agtctgccag ccgggcgggg cctgcccatg ctggccacac ggcccccatg cggccctcct
     2521 actctgcaca ggagggttta gctggctacc agagggaggg gccccaccca gcctggccac
     2581 agccagtgac cacctcccac tatgcccatg accccagcgg tatgttccgc tctcaatcct
     2641 tttcggaagc tgaaccccag ctgcccccag ctccggtccg agggggaagc agccgggagg
     2701 ctgtgcaaag gggactgaac tcgtggcagc agcagcagca gcagcagcag cagcctcgcc
     2761 cacctccacg ccagcaggaa agagcccact tggagagtct tgtagccagc aggcccagcc
     2821 ctcagccatt ggcagagacc cccatcccca gtctccctga gttcccgcga gcagcctccc
     2881 agcaggagat tgaacagtcc atcgaaacac tcaatatgct gatgctggac ctggagccag
     2941 cctccgctgc tgccccacta cacaagtccc agagtgtccc cggggcctgg ccaggggctt
     3001 ctccactctc ctcccagccc ctctctgggt cctcccgtca gtcccatcca ctgacccagt
     3061 ccagatatgg ctatatcccc agtgggcatt cgttgggaac ccctgagcca gccccacggg
     3121 cctctctgga gtctgtccct cctggcaggt cttactcacc ttatgactat cagccatgtt
     3181 tggctgggcc taaccaggat ttccattcaa agagcccagc ctcttcctcc ttgcctgcct
     3241 tccttccgac cacccacagc cctccagggc ctcagcaacc cccagcctct ctccctggcc
     3301 tcactgctca gcctctgctc tcaccaaagg aagcgacttc agacccctcc cggactccag
     3361 aggaggagcc attgaattta gaagggctgg tggcccacag ggtagcaggg gtacaggctc
     3421 gggagaagca gcctgcagag cccccagccc ctctgcggag gcgggcggcc agtgatggac
     3481 agtatgagaa ccagtctcca gaagccacat cccctcgtag ccctggggtt cgctcccctg
     3541 tccagtgtgt ctccccggag ctggctctta ccatcgctct caatcctgga gggcggccca
     3601 aagagcccca tttgcacagc tacaaggagg ccttcgagga gatggaggga acctccccga
     3661 gcagcccacc acccagtggg gtgcggtccc ccccgggtct ggccaagaca cccctgtctg
     3721 ctctgggcct gaaacctcac aacccagcgg acatcctgtt gcaccccaca ggagttacca
     3781 gaagacggat tcagccagag gaagatgagg ggaaggtggt ggtcaggctg tcagaagagc
     3841 cccggagcta tgtggagtct gtggcacgga cagcggtggc tggaccccga gctcaggact
     3901 ctgagcccaa gagctttagt gctccagcca cccaggccta tggccatgag atacccctga
     3961 ggaacgggac cctgggtggc tcctttgtct cccccagccc cctctccacc agcagcccca
     4021 tcctcagtgc tgacagcact tcagtgggga gtttcccgtc gggagagagc agtgaccagg
     4081 gtccccggac gcccacccag cctctgttgg agtctggctt ccgctcaggc agcctgggac
     4141 agcccagccc gtctgcccag agaaactacc agagctcttc tcctctcccg actgtgggca
     4201 gtagctacag cagccccgac tactcacttc agcatttcag ctcctctcca gaaagccagg
     4261 ctcgcgctca gttcagtgtg gctggcgtcc acacggtgcc tgggagccct caggcgcgcc
     4321 acagaacagt gggcaccaac actcccccta gtcctggctt cggctggcgg gccatcaatc
     4381 ccagcatggc tgcccccagc agtcccagtt tgagccatca ccagatgatg ggtccaccag
     4441 gcactggctt ccatggtagc actgtctcca gcccccagag cagtgcagcg accaccccgg
     4501 ggagccccag cctgtgtcgg cacccagcag gggtctacca ggtttctggc ctccacaaca
     4561 aagtggccac caccccgggg agtcccagcc tgggccggca ccctggggct caccaaggca
     4621 acctggcctc cggtcttcat agcaatgcaa tagccagccc tggaagcccc agcctgggcc
     4681 gtcacctcgg agggtctgga tctgtggttc ccggcagccc ctgcttggac cggcatgtgg
     4741 cctatggtgg ctattctacc ccggaggatc ggagacccac actgtcccgg cagagcagtg
     4801 cctctggcta ccaggctcct tccacgccct ccttccctgt ctcccctgcc tactaccctg
     4861 gcctgagcag ccctgccacc tccccgtcac cagactccgc agccttccgg caagggagcc
     4921 caacaccagc cttgccagag aagcgaagga tgtcagtggg agaccgggca ggcagcctcc
     4981 ccaactatgc caccatcaat gggaaggtgt cttcgcctgt cgccagcggc atgtccagtc
     5041 ccagtggggg cagcaccgtc tccttctccc acactctgcc cgacttctcc aagtactcca
     5101 tgccagacaa cagcccggag acgcgggcta aagtgaagtt tgtccaggac acttctaagt
     5161 attggtacaa gcctgagatt tccagggagc aggccatcgc gctcctcaag gaccaggagc
     5221 cgggggcctt catcatccgc gacagtcact ccttccgagg cgcgtacggg ctggccatga
     5281 aggtgtcttc gccacctcca accatcatgc agcagaataa aaaaggagac atgacccatg
     5341 agctggtcag gcattttctg atagagactg gccccagagg agtcaagctc aagggctgcc
     5401 ccaatgagcc aaacttcgga tcgctgtctg ccctggtcta ccagcactcc atcatcccat
     5461 tggccctgcc ttgcaagctg gtcattccaa accgagaccc cacagatgaa tcgaaagata
     5521 gctccggccc tgccaactca actgcagacc tgctgaaaca aggggcagcc tgcaatgtgc
     5581 tcttcgtcaa ctctgtggac atggagtcac tcactgggcc acaggccatc tctaaagcca
     5641 catctgagac gttggctgca gaccccacgc cagctgccac catcgttcac ttcaaagtct
     5701 ctgcccaggg aatcactctg actgacaacc agagaaagct ctttttcaga cgccactacc
     5761 ctctcaacac tgtcaccttc tgtgacctgg acccacagga aagaaagtgg atgaaaacag
     5821 agggtggtgc ccctgctaag ctcttcggct tcgtggcccg gaagcagggc agcaccacgg
     5881 acaacgcctg ccacctcttt gctgagcttg accccaacca gccggcctct gccatcgtca
     5941 acttcgtctc caaggtcatg ctgaatgccg gccaaaagag atgaggatcc accggatcta
     6001 gataactgat cataatcagc cata
//
