LOCUS       sequence-85993-e        2189 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 434, Emission: 
            474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2189)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2189
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1441..1450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1447..1449,1450..1452,1453..2166)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1492..1513)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1753..1772)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2100..2121
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatctcgagc tcaagcttcg aattcccatg tccaccaggt ccgtgtcctc gtcctcctac
       61 cgcaggatgt tcggcggccc gggcaccgcg agccggccga gctccagccg gagctacgtg
      121 actacgtcca cccgcaccta cagcctgggc agcgcgctgc gccccagcac cagccgcagc
      181 ctctacgcct cgtccccggg cggcgtgtat gccacgcgct cctctgccgt gcgcctgcgg
      241 agcagcgtgc ccggggtgcg gctcctgcag gactcggtgg acttctcgct ggccgacgcc
      301 atcaacaccg agttcaagaa cacccgcacc aacgagaagg tggagctgca ggagctgaat
      361 gaccgcttcg ccaactacat cgacaaggtg cgcttcctgg agcagcagaa taagatcctg
      421 ctggccgagc tcgagcagct caagggccaa ggcaagtcgc gcctggggga cctctacgag
      481 gaggagatgc gggagctgcg ccggcaggtg gaccagctaa ccaacgacaa agcccgcgtc
      541 gaggtggagc gcgacaacct ggccgaggac atcatgcgcc tccgggagaa attgcaggag
      601 gagatgcttc agagagagga agccgaaaac accctgcaat ctttcagaca ggatgttgac
      661 aatgcgtctc tggcacgtct tgaccttgaa cgcaaagtgg aatctttgca agaagagatt
      721 gcctttttga agaaactcca cgaagaggaa atccaggagc tgcaggctca gattcaggaa
      781 cagcatgtcc aaatcgatgt ggatgtttcc aagcctgacc tcacggctgc cctgcgtgac
      841 gtacgtcagc aatatgaaag tgtggctgcc aagaacctgc aggaggcaga agaatggtac
      901 aaatccaagt ttgctgacct ctctgaggct gccaaccgga acaatgacgc cctgcgccag
      961 gcaaagcagg agtccactga gtaccggaga caggtgcagt ccctcacctg tgaagtggat
     1021 gcccttaaag gaaccaatga gtccctggaa cgccagatgc gtgaaatgga agagaacttt
     1081 gccgttgaag ctgctaacta ccaagacact attggccgcc tgcaggatga gattcagaat
     1141 atgaaggagg aaatggctcg tcaccttcgt gaataccaag acctgctcaa tgttaagatg
     1201 gcccttgaca ttgagattgc cacctacagg aagctgctgg aaggcgagga gagcaggatt
     1261 tctctgcctc ttccaaactt ttcctccctg aacctgaggg aaactaatct ggattcactc
     1321 cctctggttg atacccactc aaaaaggaca cttctgatta agacggttga aactagagat
     1381 ggacaggtta tcaacgaaac ttctcagcat cacgatgacc ttgaagggga tccaccggtc
     1441 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1501 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1561 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1621 cccaccctcg tgaccaccct gtcctggggc gtgcagtgct tcgcccgcta ccccgaccac
     1681 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1741 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1801 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1861 gggcacaagc tggagtacaa ctacatcagc gacaacgtct atatcaccgc cgacaagcag
     1921 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
     1981 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2041 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     2101 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     2161 aagtaaagcg gccgcgactc tagatcata
//
