LOCUS       sequence-86032-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To create stable cell clones with high-level expression of Cas9 and 
            OCT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W
  TITLE     High-throughput screening of a CRISPR/Cas9 library for functional 
            genomics in human cells.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13166.
  PUBMED    24717434
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13166."
COMMENT     Sequence Label: pLenti-OC-IRES-BSD
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..121)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggccttattc caagcggctt cggccagtaa cgttaggggg ggggagggag aggggcggaa
       61 ttttaattaa ttatttcttt ttcttagctt gaccagcttt cttagtagca gcaggacgct
      121 tgtctccacc gagctgagag aggtcgattc ttgtttcata gagccccgta attgactgat
      181 gaatcagtgt ggcgtccagg acctcctttg tagaggtgta ccgctttctg tctatggtgg
      241 tgtcgaagta cttgaaggct gcaggcgcgc ccaagttggt cagagtaaac aagtggataa
      301 tgttttctgc ctgctccctg atgggcttat ccctgtgctt attgtaagca gaaagcacct
      361 tatcgaggtt agcgtcggcg aggatcactc ttttggagaa ttcgcttatt tgctcgatga
      421 tctcatcaag gtagtgtttg tgttgttcca cgaacagctg cttctgctca ttatcttcgg
      481 gagacccttt gagcttttca tagtggctgg ccagatacaa gaaattaacg tatttagagg
      541 gcagtgccag ctcgttacct ttctgcagct cgcccgcact agcgagcatt cgtttccggc
      601 cgttttcaag ctcaaagaga gagtacttgg gaagcttaat gatgaggtct tttttgacct
      661 ctttatatcc tttcgcctcg agaaagtcga tggggttttt ttcgaagctt gatcgctcca
      721 tgattg
//
