LOCUS       sequence-86034-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 
            with PreScission protease cleavage site linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-PreE2
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             73..96
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataaccatgg ctagctcgcg agtcgacggc
       61 ggccgcgaat tcctcgaggt tctgttccag ggtccaggtg gcggaagctt agcatccatg
      121 tcggggatcg ccctcagcag actcgcccag gagaggaaag catggaggaa agaccaccca
      181 tttggtttcg tggctgtccc aacaaaaaat cccgatggca cgatgaacct catgaactgg
      241 gagtgcgcca ttccgggaaa gaaagggact ccgtgggaag gaggcttgtt taaactacgg
      301 atgcttttca aagatgatta tccatcttcg ccaccaaaat gtaaattcga accaccatta
      361 tttcacccga atgtgtaccc ttcggggaca gtgtgcctgt ccatcttaga ggaggacaag
      421 gactggaggc cagccatcac aatcaaacag atcctattag gaatacagga acttctaaat
      481 gaaccaaata tccaagaccc agctcaagca gaggcctaca cgatttactg ccaaaacaga
      541 gtggagtacg agaaaagggt ccgagcacaa gccaagaagt ttgcgccctc atcaggatcc
      601 ggaatgtccc ctatactagg ttattggaaa attaagggcc ttgtgcaacc cactcgactt
      661 cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat
      721 aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt
      781 gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac
      841 aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt
      901 ttggatatta
//
