LOCUS       sequence-86043-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA expression construction in a 3rd generation lentiviral 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W
  TITLE     High-throughput screening of a CRISPR/Cas9 library for functional 
            genomics in human cells.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13166.
  PUBMED    24717434
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13166."
COMMENT     Sequence Label: pLenti-sgRNA-Lib
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..181
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        359..461
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(528..547)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acttgctatt tctagctcta aaacagagac gttcgaaccg cgggccctct agatcaacca
       61 ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
      121 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
      181 gaatcaacta cttagatggt attagtgacc tgtagtcgac taagttggca gcatcacccg
      241 acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc
      301 ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg
      361 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
      421 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
      481 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
      541 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
      601 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
      661 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
      721 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
      781 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
      841 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
      901 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
      961 tcgcccccgt tttcaccatg ggcaaatatt atacgca
//
