LOCUS       sequence-86051-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcription of guide RNA (gRNA) in maize cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Liang Z, Zhang K, Chen K, Gao C
  TITLE     Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas 
            System.
  JOURNAL   J Genet Genomics. 2014 Feb 20;41(2):63-8. doi: 
            10.1016/j.jgg.2013.12.001. Epub 2013 Dec 14.
  PUBMED    24576457
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jgg.2013.12.001"; journalName: "J Genet Genomics"; date: 
            "2014-02-20- 20"; volume: "41"; issue: "2"; pages: "63-8"
COMMENT     Sequence Label: pZmU3-gRNA
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(94..169)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattgc
       61 ccttagccac cgtaggatcc ggtaccaaaa aaagcaccga ctcggtgcca ctttttcaag
      121 ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaacg tgtcttctgt
      181 gttgaagaca gtgctgtgag tcaccaaagc taaattttta agctggcttc tacgcatgtg
      241 agcaaccgag atggcactaa ccggtgggac tggccaacat actgcgtcct gggtcggccc
      301 attctggtat cctacatgtg ggcttcgggt ccagccaatc gaggcagtag tagcttgatg
      361 cctcacctgg atgaaaaaaa ggaaagactg ataacctcag ctgatcccta gacgaggcac
      421 aaagctcaat ctttagccca tgtgcaatgc tggatgttgc cgccacatgg ttgctgttga
      481 tcttggcagc gaagcgaggg tggcctgcag atcagtgatg atgctcagct taggaaatct
      541 taatacaagc tcgaaagcat ttgggtaatg gtggctaaac tactagtaat tacactctac
      601 gctcaccttt tagtcacctt cagaaatgta gtttctgcat agtgcctcct tctgaactat
      661 tggctcattt ttttttgttt ctgtcgtcat cacactgttg ggcccgtgtt gattcaaggt
      721 agttgaggtt tctggcatca tcacattttt ggccagcggt gagactaaaa tcaagcagtg
      781 gatgatgatg gcatgcggtg ttagttttga agatctacta gctacataca tcgcagaaat
      841 gcagaatagc atgagcatcg gtcacaactc acaatcacaa ggcaccggag ctgcatagac
      901 ctgaagtggg catccaaatt aatccatgct ttaccaagat acttagatgg aattcaaggg
      961 caattctgca gatatccatc acactggcgg ccgctcgagc
//
