LOCUS       sequence-86110-e        4282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Theophylline-inducible expression in single-copy integrating vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4282)
  TITLE     JSeeliger Lab riboswitch plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRiboI SapNde
FEATURES             Location/Qualifiers
     source          1..4282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1605..1624)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1616..2431
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1689..1708)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2763..3351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3252..3271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      3923..3969
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 accagcggag cgggcgacgg gaatcgaacc cgcgtagcta gtttggaaga atgggtgtct
       61 gccgaccaca tatatgggcc ggtcaagata ggtttttacc ccctctcggc tgcatcctct
      121 aagtggaaag aaattgcagg tcgtagaagc gcgttgaagc ctgagagttg cacaggagtt
      181 gcaacccggt agccttgttc acgacgagag gagacctagt tggcacgtcg cggatgggga
      241 tcgctgaaga ctcagcgcag cgggaggatc caagcctcat acgtcaaccc gcaggacggt
      301 gtgaggtact acgcgctgca gacctacgac aacaagatgg acgccgaagc ctggctcgcg
      361 ggcgagaagc ggctcatcga gatggagacc tggacccctc cacaggaccg ggcgaagaag
      421 gcagccgcca gcgccatcac gctggaggag tacacccgga agtggctcgt ggagcgcgac
      481 ctcgcagacg gcaccaggga tctgtacagc gggcacgcgg agcgccgcat ctacccggtg
      541 ctaggtgaag tggcggtcac agagatgacg ccagctctgg tgcgtgcgtg gtgggccggg
      601 atgggtagga agcacccgac tgcccgccgg catgcctaca acgtcctccg ggcggtgatg
      661 aacacagcgg tcgaggacaa gctgatcgca gagaacccgt gccggatcga gcagaaggca
      721 gccgatgagc gcgacgtaga ggcgctgacg cctgaggagc tggacatcgt cgccgctgag
      781 atcttcgagc actaccggat cgcggcatac atcctggcgt ggacgagcct ccggttcgga
      841 gagctgatcg agcttcgccg caaggacatc gtggacgacg gcatgacgat gaagctccgg
      901 gtgcgccgtg gcgcttcccg cgtggggaac aagatcgtcg ttggcaacgc caagaccgtc
      961 cggtcgaagc gtcctgtgac ggttccgcct cacgtcgcgg agatgatccg agcgcacatg
     1021 aaggaccgta cgaagatgaa caagggcccc gaggcattcc tggtgaccac gacgcagggc
     1081 aaccggctgt cgaagtccgc gttcaccaag tcgctgaagc gtggctacgc caagatcggt
     1141 cggccggaac tccgcatcca cgacctccgc gctgtcggcg ctacgttcgc cgctcaggca
     1201 ggtgcgacga ccaaggagct gatggcccgt ctcggtcaca cgactcctag gatggcgatg
     1261 aagtaccaga tggcgtctga ggcccgcgac gaggctatcg ctgaggcgat gtccaagctg
     1321 gccaagacct cctgaaacgc aaaaagcccc cctcccaagg acactgagtc ctaaagaggg
     1381 gggtttcttg tcagtacgcg aagaaccacg cctggccgcg agcgccagca ccgccgctct
     1441 gtgcggagac ctgggcacca gccccgccgc cgccaggagc attgccgttc ccgccagcta
     1501 gcaacaaagc gacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     1561 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     1621 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga
     1681 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1741 cttgtatggg aagccccatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1801 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     1861 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1921 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1981 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     2041 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     2101 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     2161 gcataatctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     2221 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     2281 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     2341 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     2401 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     2461 gcagagcatt acgctgactt gacgggacgg cggctttgtt gaataaatcg aacttttgct
     2521 gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa
     2581 aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc
     2641 actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac accttcttca
     2701 cgaggcagac ctcactagtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2761 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2821 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2881 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2941 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3001 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3061 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3121 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     3181 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3241 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3301 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3361 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3421 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3481 cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtaccagatc tttaaatcta
     3541 gaggtgacca caacgcgccc gctttgatcg gggacgtctg cggccgacca tttacgggtc
     3601 ttgttgtcgt tggcggtcat gggccgaaca tactcacccg gatcggaggg ccgaggacaa
     3661 ggtcgaacga ggggcatgac ccggtgcggg gcttcttgca ctcggcatag gcgagtgcta
     3721 agaataacgt tggcactcgc gaccgggata cgactcacta taggtaccgg tgataccagc
     3781 atcgtcttga tgcccttggc agcaccctgc taaggaggca acaagatgga agagcatcat
     3841 atgtatccat ggatccagct gcagaattcg aagcttatcg atgtcgacgt agttaactag
     3901 cgtacgatcg actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     3961 tcgttttatg ccatcatggc cgcggtgatc agctagagcc gtgaacgaca gggcgaacgc
     4021 cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct tgtcccggtc
     4081 tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg ttcgcgcagg
     4141 cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg cgttgcaacc
     4201 gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa gaaaaatggc
     4261 cagagggcga aaacaccctc tg
//
