LOCUS       sequence-86148-e        1177 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of IFIT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K
  TITLE     tRNA binding, structure, and localization of the human 
            interferon-induced protein IFIT5.
  JOURNAL   Mol Cell. 2013 Feb 21;49(4):743-50. doi: 
            10.1016/j.molcel.2012.12.015. Epub 2013 Jan 11.
  PUBMED    23317505
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.12.015"; journalName: "Mol Cell"; date: 
            "2013-02-21- 21"; volume: "49"; issue: "4"; pages: "743-50"
COMMENT     Sequence Label: pET28a(+) 6xHis-TEV-IFIT2
FEATURES             Location/Qualifiers
     source          1..1177
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..149
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..212
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             228..248
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
       61 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      121 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
      181 atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcagagaa aacctgtatt
      241 ttcagagtga gaacaataag aattccttgg agagcagcct acggcaacta aaatgccatt
      301 tcacctggaa cttgatggag ggagaaaact ccttggatga ttttgaagac aaagtatttt
      361 accggactga gtttcagaat cgtgaattca aagccacaat gtgcaaccta ctggcctatc
      421 taaagcacct caaagggcaa aacgaggcag ccctggaatg cttacgtaaa gctgaagagt
      481 taatccagca agagcatgct gaccaggcag aaatcagaag tctggtcacc tggggaaact
      541 atgcctgggt ctactatcac atgggccgac tctcagacgt tcagatttat gtagacaagg
      601 tgagacatgt ctgtgagaag ttttccagtc cctatagaat tgagagtcca gagcttgact
      661 gtgaggaagg gtggacacgg ttaaagtgtg gaggaaacca aaatgaaaga gcgaaggtgt
      721 gctttgagaa ggctctggaa aagaagccaa agaacccaga attcacctct ggactggcaa
      781 tagcaagcta ccgtctggac aactggccac catctcagaa cgccattgac cctctgaggc
      841 aagccattcg gctgaatcct gacaaccagt accttaaagt cctcctggct ctgaagcttc
      901 ataagatgcg tgaagaaggt gaagaggaag gtgaaggaga gaagttagtt gaagaagcct
      961 tggagaaagc cccaggtgta acagatgtac ttcgcagtgc agccaagttt tatcgaagaa
     1021 aagatgagcc agacaaagcg attgaactgc ttaaaaaggc tttagaatac ataccaaaca
     1081 atgcctacct gcattgccaa attgggtgct gctatagggc aaaagtcttc caagtaatga
     1141 atctaagaga gaatggaatg tatgggaaaa gaaagtt
//
