LOCUS       sequence-86326-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human TDG-K330A C-terminally fused to GyrA 
            intein and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pTXG-hTDG-K330A
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..640)
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
ORIGIN
        1 gggacattcc ggatccgagg cctgagttca gaccggtgag gccagtagcg tggctgacga
       61 acccgttcgt gataaacgcg tggtctgccg tgtcgacacg aaggctatac accggctgca
      121 cgccggcgtc ggtgacactg gcgactttcg cgtagtagaa ccgcccgtcg gtcagctcgt
      181 cggcgatagc ttgggcgtcc gggtctcggt ggtgtgcttc caagaaacgc accagtccag
      241 ggacgccgac tgtgtaggtt gtgggcgcaa attcgggttt cccgcgggca aaacctgcac
      301 agtcgacgct gaatgcgctg cgttgaatca ccgcgtaatc gcccggcttg atttcgtcga
      361 tcagcttcca cagcagggtc ggcaccccgg cgacgtcgac caaacacaac aacgggtggt
      421 tcgcggtgcc cgtcacacgc agaccttcga ccgtacgcac cgtgtacacc ggatgctcgc
      481 cggagtggaa cagccggtcg gcgagcacgg gattgccatg ccggtcaagg actttcaggt
      541 cgatggcgtt gtcactgttg ggccgcgcac ccggcacgat gtcggcgatg cgtaccgact
      601 cgccctcggg tagggcaact agtgcatctc cgcagatgca catctcgagg aattcagcat
      661 ggctttcttc ttcctgttct tgtgttccac agtgattact aaaggaagga atttggtctg
      721 taaatgactg ggtcatccac tgcccattag gaatgccact gaaagctgat tctcctctta
      781 actccacgct ctcaattagc ccatttgaag agaagccaca aggttcactg ctgcatggat
      841 tttctccgta agcaccacca tatgctgcct cataacctg
//
