LOCUS       sequence-86350-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector with vacuole marker (gamma-TIP) - ECFP for tagged
            fluorescent protein (N-ter-EYFP) of plant gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood1 JL
  TITLE     The Plant Glycosyltransferase Clone Collection for Functional 
            Genomics.
  JOURNAL   Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
  PUBMED    24905498
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Jun 6. doi: 10.1111/tpj.12577."
COMMENT     Sequence Label: pBullet-vac-n
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(34..55)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(190..702,703..705,706..708))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     383..402
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     642..663
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
       61 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
      121 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      181 gctgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt tcttctgctt
      241 gtcggcggtg atatagacgt tgtggctgat gtagttgtac tccagcttgt gccccaggat
      301 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      361 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
      421 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
      481 ggggtagcgg ctgaagcact gcacgcccca ggtcagggtg gtcacgaggg tgggccaggg
      541 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
      601 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      661 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg atcccccggg
      721 gtagtctgtg gttgggagct gctcgtgtgt ggtgttgatg aagaaaactt cgtagatgag
      781 tccagcgatt ccaccgcc
//
