LOCUS       sequence-86358-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector with vacuole marker (gamma-TIP) - ECFP for tagged
            fluorescent protein (C-ter-EYFP) of plant gene .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood1 JL
  TITLE     The Plant Glycosyltransferase Clone Collection for Functional 
            Genomics.
  JOURNAL   Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
  PUBMED    24905498
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Jun 6. doi: 10.1111/tpj.12577."
COMMENT     Sequence Label: pBullet-vac-c
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(495..619)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(653..669)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(654..670)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(673..719)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(708..728)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
       61 atataaatgt caggctccct tatacacagc cagtctgcag gtcgatacag tagaaattac
      121 agaaacttta tcacgtttag taagtataga ggctgaaaat ccagatgaag ccgaacgact
      181 tgtaagagaa aagtataaga gttgtgaaat tgttcttgat gcagatgatt ttcaggacta
      241 tgacactagc gtatatgaat aggtagatgt ttttattttg tcacacaaaa aagaggctcg
      301 cacctctttt tcttatttct ttttatgatt taatacggca ttgaggacaa tagcgagtag
      361 gctggatacg acgattccgt ttgagaagaa catttggaag gctgtcggtc gactaagttg
      421 gcagcatcac ccgaagaaca tttggaaggc tgtcggtcga ctacaggtca ctaataccat
      481 ctaagtagtt gattcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
      541 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
      601 cttttttgta caaacttgtt gatgggctgc aggaattcga tatcaagctt atcgataccg
      661 tcgacctcga ggtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
      721 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
      781 gctttgaaga cgtggttgga acgtcttctt tt
//
