LOCUS       sequence-86366-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 
            with PreScission protease cleavage site linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-PreE2
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(517..540)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             584..606
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    621..645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(645..664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(646..664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aggggacatt ccggatcctg atgagggcgc aaacttcttg gcttgtgctc ggaccctttt
       61 ctcgtactcc actctgtttt ggcagtaaat cgtgtaggcc tctgcttgag ctgggtcttg
      121 gatatttggt tcatttagaa gttcctgtat tcctaatagg atctgtttga ttgtgatggc
      181 tggcctccag tccttgtcct cctctaagat ggacaggcac actgtccccg aagggtacac
      241 attcgggtga aataatggtg gttcgaattt acattttggt ggcgaagatg gataatcatc
      301 tttgaaaagc atccgtagtt taaacaagcc tccttcccac ggagtccctt tctttcccgg
      361 aatggcgcac tcccagttca tgaggttcat cgtgccatcg ggattttttg ttgggacagc
      421 cacgaaacca aatgggtggt ctttcctcca tgctttcctc tcctgggcga gtctgctgag
      481 ggcgatcccc gacatggatg ctaagcttcc gccacctgga ccctggaaca gaacctcgag
      541 gaattcgcgg ccgccgtcga ctcgcgagct agccatggtt atatctcctt cttaaagtta
      601 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      661 attaatttcg cgggatcgag atccccggtt taaaccggga attaattccg atccccaatt
      721 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt
      781 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca a
//
