LOCUS       sequence-86397-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of DHX36.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Sexton AN, Collins K
  TITLE     The 5' guanosine tracts of human telomerase RNA are recognized by 
            the G-quadruplex binding domain of the RNA helicase DHX36 and 
            function to increase RNA accumulation.
  JOURNAL   Mol Cell Biol. 2011 Feb;31(4):736-43. doi: 10.1128/MCB.01033-10. 
            Epub 2010 Dec 13.
  PUBMED    21149580
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01033-10"; journalName: "Mol Cell Biol"; date: 
            "2011-02"; volume: "31"; issue: "4"; pages: "736-43"
COMMENT     Sequence Label: pcDNA3.1 TAP DHX36
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             144..317
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             318..491
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             549..569
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             579..656
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagagc tgccaccatg aaaaccgcgg
      121 ctcttgcgca acacgatgaa gccgtggaca acaaattcaa caaagaacaa caaaacgcgt
      181 tctatgagat cttacattta cctaacttaa acgaagaaca acgaaacgcc ttcatccaaa
      241 gtttaaaaga tgacccaagc caaagcgcta accttttagc agaagctaaa aagctaaatg
      301 atgctcaggc gccgaaagta gacaacaaat tcaacaaaga acaacaaaac gcgttctatg
      361 agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa
      421 aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta aatggtgctc
      481 aggcgccgaa agtagacgcg aattccgcgg ggaagtcaac cgattatgat attccaacta
      541 ctgctagcga gaatttgtat tttcagggtt ccatggaaaa gagaagatgg aaaaagaatt
      601 tcatagccgt ctcagcagcc aaccgcttta agaaaatctc atcctccggg gcacttggag
      661 caggtgctaa gcttatgagt tatgactacc atcagaactg gggccgtgat gggggtcccc
      721 gcagctccgg tgggggctat ggaggggggc cagcaggggg tcatggaggt aaccgaggct
      781 ccggaggagg cggcggcggc ggagggggtg gtcgaggcgg caggggccgg catcccgggc
      841 acctgaaagg ccgcgaaatc ggcatgtggt acgcgaaaaa acaggggcag aagaacaagg
      901 aagcggagag gcaagagaga gctgtagtac acatggatga acgacgagaa gaacaaattg
      961 tacagttact gaattctgtt caagcgaaga atgataaaga gtcagaagca cagatatcct
     1021 ggtttgctcc tgaggatcat ggatacggta ctgaagtttc tactaa
//
