LOCUS       sequence-86420-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for 
            tagged fluorescent protein (N-ter-EYFP) of plant gene .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood1 JL
  TITLE     The Plant Glycosyltransferase Clone Collection for Functional 
            Genomics.
  JOURNAL   Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
  PUBMED    24905498
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Jun 6. doi: 10.1111/tpj.12577."
COMMENT     Sequence Label: pBullet-er-n
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(18..728,729..731,732..734))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(60..81)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     409..428
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     668..689
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcgtcatgag atctcttctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
       61 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
      121 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
      181 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
      241 ttggccttga tgccgttctt ctgcttgtcg gcggtgatat agacgttgtg gctgatgtag
      301 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
      361 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
      421 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
      481 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccccaggtc
      541 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
      601 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
      661 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
      721 cccttgctca ccatggatcc gcactccttg cgaggttgcc ccttgactag ctgcgtataa
      781 gcaaggtaga aaacagccac cacgaacaaa ccctcctgta ccttcattcc caaacaaaga
      841 tctgggctgt cctctccaaa tgaaatgaac ttccttatat agaggaa
//
