LOCUS       sequence-86445-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of full length human Uba2 with Cterminal His-tag in 
            E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Werner A, Moutty MC, Moller U, Melchior F
  TITLE     Performing in vitro sumoylation reactions using recombinant enzymes.
  JOURNAL   Methods Mol Biol. 2009;497:187-99. doi: 
            10.1007/978-1-59745-566-4_12.
  PUBMED    19107418
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2009"; volume: "497:187-99. doi"; pages: " 
            10.1007/978-1-59745-566-4_12"
COMMENT     Sequence Label: pET28b-hUba2-His
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac
       61 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata taccatgggg atggcactgt cgcgggggct gccccgggag ctggctgagg
      181 cggtggccgg gggccgggtg ctggtggtgg gggcgggcgg catcggctgc gagctcctca
      241 agaatctcgt gctcaccggt ttctcccaca tcgacctgat tgatctggat actattgatg
      301 taagcaacct caacagacag tttttgtttc aaaagaaaca tgttggaaga tcaaaggcac
      361 aggttgccaa ggaaagtgta ctgcagtttt acccgaaagc taatatcgtt gcctaccatg
      421 acagcatcat gaaccctgac tataatgtgg aatttttccg acagtttata ctggttatga
      481 atgctttaga taacagagct gcccgaaacc atgttaatag aatgtgcctg gcagctgatg
      541 ttcctcttat tgaaagtgga acagctgggt atcttggaca agtaactact atcaaaaagg
      601 gtgtgaccga gtgttatgag tgtcatccta agccgaccca gagaaccttt cctggctgta
      661 caattcgtaa cacaccttca gaacctatac attgcatcgt ttgggcaaag tacttgttca
      721 accagttgtt tggggaagaa gatgctgatc aagaagtatc tcctgacaga gctgaccctg
      781 aagctgcctg ggaaccaacg gaagccgaag ccagagctag agcatctaat gaagatggtg
      841 acattaaacg tatttctact aaggaatggg ctaaatcaac tggatatgat ccagttaaac
//
