LOCUS       sequence-86477-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli expression of Cterminal domain of human RanGAP1 with 
            Nterminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Flotho A, Werner A, Winter T, Frank AS, Ehret H, Melchior F
  TITLE     Recombinant reconstitution of sumoylation reactions in vitro.
  JOURNAL   Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5.
  PUBMED    22350878
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2012"; volume: "832:93-110. doi"; pages: " 
            10.1007/978-1-61779-474-2_5"
COMMENT     Sequence Label: pET23a-His-hRanGAP1tail
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             635..652
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(705..723)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      719..766
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac atatggctca ccaccaccac caccacggtg gatcccctca
       61 gcagcgaggg cagggagaga agtcagccac gccctcacgg aagattctgg accctaacac
      121 tggggagcca gctcccgtgc tgtcctcccc acctcctgca gacgtctcca ccttcctggc
      181 ttttccctct ccagagaagc tgctgcgcct agggcccaag agctccgtgc tgatagccca
      241 gcagactgac acgtctgacc ccgagaaggt ggtctctgcc ttcctaaagg tgtcatctgt
      301 gttcaaggac gaagctactg tgaggatggc agtgcaggat gcagtagatg ccctgatgca
      361 gaaggctttc aactcctcgt ccttcaactc caacaccttc ctcaccaggc tcctcgtgca
      421 catgggtctg ctcaagagtg aagacaaggt caaggccatt gccaacctgt acggccccct
      481 gatggcgctg aaccacatgg tgcagcagga ctatttcccc aaggcccttg cacccctgct
      541 gctggcgttc gtgaccaagc ccaacagcgc cctggaatcc tgctccttcg cccgccacag
      601 tctgctgcag acgctgtaca aggtctagct cgagcaccac caccaccacc actgagatcc
      661 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      721 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      781 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      841 gtggttacgc gcagcgtgac cgctacactt gccagc
//
