LOCUS       sequence-86505-e        1749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically encoded caged Ca2+.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Fukuda N, Matsuda T, Nagai T
  TITLE     Optical control of the ca(2+) concentration in a live specimen with 
            a genetically encoded ca(2+)-releasing molecular tool.
  JOURNAL   ACS Chem Biol. 2014 May 16;9(5):1197-203. doi: 10.1021/cb400849n. 
            Epub 2014 Mar 24.
  PUBMED    24625002
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb400849n";
            journalName: "ACS Chem Biol"; date: "2014-05-16- 16"; volume: "9"; 
            issue: "5"; pages: "1197-203"
COMMENT     Sequence Label: msEGFP-PACR_pcDNA3
FEATURES             Location/Qualifiers
     source          1..1749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1666..1743
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagcgc
      721 atgcatgacc aactgacaga agagcagatt gcagagttca aagaagcctt ctcattattc
      781 gacaaggatg gggacggcac catcaccaca aaggaacttg gcaccgttat gaggtcgctt
      841 ggactcgagc tggccaccac tctagagcgc atcgagaaga acttcgtgat caccgacccc
      901 cgcctgcccg acaaccccat catcttcgcc tccgactcct tcctgcagct gaccgagtac
      961 tcccgcgagg agatcctggg ccgcaactgc cgcttcctgc agggccccga gaccgaccgc
     1021 gccaccgtgc gcaagatccg cgacgccatc gacaaccaga ccgaggtgac cgtgcagctg
     1081 atcaactaca ccaagtccgg caagaagttc tggaacctgt tccacctgca gcccatgcgc
     1141 gaccagaagg gcgacgtgca gtacttcatc ggcgtgcagc tggacggcac cgagcacgtg
     1201 cgcgacgccg ccgagcgcga gggcgtgatg ctgatcaaga agaccgccga gaacatcgac
     1261 gaggccgcca aggagctgcc cgacgccaac ctgcgccccg aagatctgtg ggccaaccac
     1321 ggtacccaaa acccaacgga agcagaattg caggatatga tcaatgaagt cgatgctgat
     1381 ggcaatggaa cgatttactt tcctgaattt cttactatga tggctagaaa aatgaaggac
     1441 acagacagcg aagaggaaat ccgagaagca ttccgtgttt ttgacaagga tgggaacggc
     1501 tacatcagcg ctgctgaatt acgtcacgtc atgacaaacc tcggggagaa gttaacagat
     1561 gaagaagttg atgaaatgat aagggaagca gatatcgatg gtgatggcca agtaaactat
     1621 gaagagtttg tacaaatgat gacagcaaag gggggaggtg gctccaagag gcgctggaag
     1681 aaaaacttca ttgccgtcag cgctgccaac cggttcaaga agatctccag ctccggggca
     1741 ctggagctc
//
