LOCUS       sequence-86563-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-HA tagged human MEK1 from bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pGEX6P1-HA-MEK1
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..42
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             88..114
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 catcctccaa aatcggatct ggaagttctg ttccaggggc ccctgggatc cccgaattcc
       61 cgggtcgacg atattgccgc caccatgtac ccatacgatg ttccagatta cgctcccaag
      121 aagaagccga cgcccatcca gctgaacccg gcccccgacg gctctgcagt taacgggacc
      181 agctctgcgg agaccaactt ggaggccttg cagaagaagc tggaggagct agagcttgat
      241 gagcagcagc gaaagcgcct tgaggccttt cttacccaga agcagaaggt gggagaactg
      301 aaggatgacg actttgagaa gatcagtgag ctgggggctg gcaatggcgg tgtggtgttc
      361 aaggtctccc acaagccttc tggcctggtc atggccagaa agctaattca tctggagatc
      421 aaacccgcaa tccggaacca gatcataagg gagctgcagg ttctgcatga gtgcaactct
      481 ccgtacatcg tgggcttcta tggtgcgttc tacagcgatg gcgagatcag tatctgcatg
      541 gagcacatgg atggaggttc tctggatcaa gtcctgaaga aagctggaag aattcctgaa
      601 caaattttag gaaaagttag cattgctgta ataaaaggcc tgacatatct gagggagaag
      661 cacaagatca tgcacagaga tgtcaagccc tccaacatcc tagtcaactc ccgtggggag
      721 atcaagctct gtgactttgg ggtcagcggg cagctcatcg actccatggc caactccttc
      781 gtgggcacaa ggtcctacat gtcgccagaa agactccagg ggactcatta ctctgtgcag
      841 tcagacatct ggagcatggg actgtctctg gtagagatgg cggttgggag gtatcccatc
      901 cctcctccag atgccaagga gctggagctg atgtttggg
//
