LOCUS       sequence-86578-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows expression of shRNA from U6 promoter and a rescue cDNA from a
            doxycycline (Tet) inducible promoter in the same plasmid. Contains 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Ma HT, On KF, Tsang YH, Poon RY
  TITLE     An inducible system for expression and validation of the specificity
            of short hairpin RNA in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2007  . 35(4):e22.
  PUBMED    17234679
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2007  . 35(4):e22."
COMMENT     Sequence Label: pKAR1/Pur
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        96..595
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(131..154)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     528..547
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(620..639)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cgtcctggcc ggaccacgct atcttgtgca aggtccccgg ccccggacgc gcgctccatg
       61 agcagagcgc ccgccgccga ggcgaagctt ctaccgggta ggggaggcgc ttttcccaag
      121 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
      181 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
      241 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
      301 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
      361 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
      421 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
      481 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
      541 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
      601 cagcccaagc tagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
      661 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
      721 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
      781 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
      841 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
      901 catgg
//
