LOCUS       sequence-86589-e        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GLS2A-c007
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgatt
      121 cctgattttg aggagttcac gggccatgtg gatcgcatct ttgaggatgt caaagagctc
      181 actggaggca aagtggcagc ctacatccct cagctggcca agtcaaaccc agacctgtgg
      241 ggtgtctccc tgtgcactgt ggatggtcaa cggcactctg tgggccacac aaagatcccc
      301 ttctgcctgc agtcctgtgt gaagcccctc acctatgcca tctccataag caccctaggc
      361 actgactacg tgcacaagtt tgtgggcaaa gagccaagtg gcctgcgcta caacaagctc
      421 tccctcaatg aggaaggaat cccccataac cccatggtca atgctggtgc cattgttgtc
      481 agctccctga tcaagatgga ctgtaacaaa gcagagaagt ttgattttgt gttgcagtat
      541 ctcaacaaaa tggctgggaa tgaatacatg ggtttcagca atgccacatt ccagtcagag
      601 aaggaaacag gggatcggaa ttatgccatc ggctattatc tcaaggaaaa gaagtgcttt
      661 cctaaggggg tggacatgat ggctgccctt gatctctact tccagctgtg ttctgtggag
      721 gtcacttgtg aatcaggcag tgtcatggca gccaccctcg ccaacggtgg gatctgcccc
      781 atcacaggcg agagtgtgct gagtgctgaa gcagtgcgca acaccctcag cctcatgcat
      841 tcctgcggca tgtatgactt ctctggccag tttgccttcc acgtgggcct gccagccaag
      901 tcagctgtat caggagccat cctcctggtg gtacccaatg tcatgggaat gatgtgcctg
      961 tcacccccat tggacaagct ggggaacagc cataggggga ccagcttctg ccagaagttg
     1021 gtgtctctct tcaatttcca caactatgac aacctgagg
//
