LOCUS       sequence-86596-e        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MTRA-c034
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgaaaac cctgcgggat
      121 gagatcaatg ccattctgca gaagaggatt atggtgctgg atggagggat ggggaccatg
      181 atccagcggg agaagctaaa cgaagaacac ttccgaggtc aggaatttaa agatcatgcc
      241 aggccgctga aaggcaacaa tgacatttta agtataactc agcctgatgt catttaccaa
      301 atccataagg aatacttgct ggctggggca gatatcattg aaacaaatac ttttagcagc
      361 actagtattg cccaagctga ctatggcctt gaacacttgg cctaccggat gaacatgtgc
      421 tctgcaggag tggccagaaa agctgccgag gaggtaactc tccagacagg aattaagagg
      481 tttgtggcag gggctctggg tccgactaat aagacactct ctgtgtcccc atctgtggaa
      541 aggccggatt ataggaacat cacatttgat gagcttgttg aagcatacca agagcaggcc
      601 aaaggacttc tggatggcgg ggttgatatc ttactcattg aaactatttt tgatactgcc
      661 aatgccaagg cagccttgtt tgcactccaa aatctttttg aggagaaata tgctccccgg
      721 cctatcttta tttcagggac gatcgttgat aaaagtgggc ggactctttc cggacagaca
      781 ggagagggat ttgtcatcag cgtgtctcat ggagaaccac tctgcattgg attaaattgt
      841 gctttgggtg cagctgaaat gagacctttt attgaaataa ttggaaaatg tacaacagcc
      901 tatgtcctct gttatcccaa tgcaggtctt cccaacacct ttggtgacta tgatgaaacg
      961 ccttctatga tggccaagca cctaaaggat tttgctatgg atggcttggt caatatagtt
     1021 ggaggatgct gtgggtcaac accagatcat atcagggaaa ttgctgaagc t
//
