LOCUS       sequence-86607-e        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBSA-c032
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgccttc tgagaccccc
      121 caggcagaag tggggcccac aggctgcccc caccgctcag ggccacactc ggcgaagggg
      181 agcctggaga aggggtcccc agaggataag gaagccaagg agcccctgtg gatccggccc
      241 gatgctccga gcaggtgcac ctggcagctg ggccggcctg cctccgagtc cccacatcac
      301 cacactgccc cggcaaaatc tccaaaaatc ttgccagata ttctgaagaa aatcggggac
      361 acccctatgg tcagaatcaa caagattggg aagaagttcg gcctgaagtg tgagctcttg
      421 gccaagtgtg agttcttcaa cgcgggcggg agcgtgaagg accgcatcag cctgcggatg
      481 attgaggatg ctgagcgcga cgggacgctg aagcccgggg acacgattat cgagccgaca
      541 tccgggaaca ccgggatcgg gctggccctg gctgcggcag tgaggggcta tcgctgcatc
      601 atcgtgatgc cagagaagat gagctccgag aaggtggacg tgctgcgggc actgggggct
      661 gagattgtga ggacgcccac caatgccagg ttcgactccc cggagtcaca cgtgggggtg
      721 gcctggcggc tgaagaacga aatccccaat tctcacatcc tagaccagta ccgcaacgcc
      781 agcaaccccc tggctcacta tgacaccacc gctgatgaga tcctgcagca gtgtgatggg
      841 aagctggaca tgctggtggc ttcagtgggc acgggcggca ccatcacggg cattgccagg
      901 aagctgaagg agaagtgtcc tggatgcagg atcattgggg tggatcccga agggtccatc
      961 ctcgcagagc cggaggagct gaaccagacg gagcagacaa cctacgaggt ggaagggatc
     1021 ggctacgact tcatccccac ggtgctggac aggacggtgg tggacaagtg gttcaagagc
     1081 aacgatgaga
//
