LOCUS       sequence-8661-ex        3284 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, 
            Greenblatt JF, Dilworth FJ, Rudnicki MA
  TITLE     Pax7 activates myogenic genes by recruitment of a histone 
            methyltransferase complex.
  JOURNAL   Nat Cell Biol. 2008 Jan . 10(1):77-84.
  PUBMED    18066051
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Jan . 10(1):77-84."
COMMENT     Sequence Label: pBRIT Pax7 216C
FEATURES             Location/Qualifiers
     source          1..3284
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             331..747
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     677..699
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     715..731
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    758..791
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        798..816
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             1750..1821
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    1839..1872
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1884..1904)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1889..2218
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2069..2204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2228..2827
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2228..2247)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2724..2744
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     gap             2828..2876
                     /estimated_length=49
ORIGIN
        1 tgcgtctgta ctagtagcta ctagctcgta tctggcggac ccgtggtgga actgacgagt
       61 tctgaacanc gggcgcaacc ntgggagacg tcccagggac tttggggccg tttttgtggc
      121 ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta
      181 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac
      241 cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg
      301 actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc cttaagtttg
      361 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
      421 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
      481 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
      541 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
      601 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
      661 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
      721 ccagccctca ctccttctct aggcgccggc cggatctata acttcgtata atgtatgcta
      781 tacgaagtta tggatctaat taaccctcac taaagggaac aaaagctgga gctccaccgc
      841 ggtggcggcc gctctagccc gggcggatcc gccaccatgc gccgcagtcg gaccacgttc
      901 acagccgagc agctggagga gctagagaag gcctttgaga ggacccacta cccggacatc
      961 tacacccggg aggagctggc acagaggacc aagctcacgg aggcacgcgt ccaggtctgg
     1021 ttcagtaacc ggcgtgcccg ctggcgcaag caggcaggag ctaaccagct ggccgccttc
     1081 aaccaccttc tgccgggagg tttcccaccc accggcatgc ccacgctgcc accctaccag
     1141 ctgccggact ctacctaccc caccaccacc atctcccaag atgggggcag cacagtacac
     1201 aggccccagc cccttccgcc atcaaccatg catcagggtg ggctggctgc ggccgctgca
     1261 gcagcagaca ccagctctgc ctacggagcc cgccacagct tctccagcta ctctgacagc
     1321 ttcatgaacc ctggggctcc ctccaaccac atgaaccctg tcagcaatgg cctgtctcct
     1381 caggtcatga gcatccttag caacccgagt gccgtgcctc cacagcccca ggccgacttc
     1441 tccatctccc cgctgcatgg aggcctggac tcggcttcct ccatctcagc cagctgcagc
     1501 caacgggccg actccatcaa gccaggagac agcttgccca cgtcccagtc ttactgccca
     1561 cccacctaca gcaccactgg ctacagtgtg gaccctgtgg ctggctacca gtacagccag
     1621 tatggccaaa ctgctgttga ttacctggcc aaaaacgtga gcctgtccac acagcgccgt
     1681 atgaagcttg gggaacactc cgctgtgctg ggacttcttc ctgtggaaac gggacaagcc
     1741 tacctcgagg actacaagga tgacgatgac aaggattaca aagacgacga tgataaggac
     1801 tataaggatg atgacgacaa ataatagtct agagatccat aacttcgtat aatgtatgct
     1861 atacgaagtt atctcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     1921 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     1981 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2041 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     2101 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     2161 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     2221 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     2281 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     2341 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     2401 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     2461 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     2521 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     2581 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     2641 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     2701 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     2761 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     2821 tgcctgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatcg
     2881 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     2941 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa atacataact
     3001 gagaatagag aagttcagat caggtcagga acagatggaa cagctgaata tgggccaaac
     3061 aggatatctg tggtaagcag ttcctgcccg gctcagggcc aagaacagat ggaacagctg
     3121 aatatgggcc aacagaatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     3181 agatggtccc caaatgcggt ccagccctca gcagtttcta gagaacatca gatgtttcca
     3241 gggtgcccaa gactgaatga cctgtgctat gactacatca gagc
//
