LOCUS       sequence-86617-e        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZFYVE9C-c009
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat catcatcatt
       61 cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgaat cttattcctg
      121 aagatggcct tcctcccatt ctcatctcca ctggtgtaaa aggagactat gctgtggaag
      181 agaaaccatc acagatttca gtaatgcagc agttggagga tggtggccct gacccacttg
      241 tatttgtttt aaatgcaaat ttgttgtcaa tggttaaaat tgtaaattat gtgaacagga
      301 agtgctggtg tttcacaacc aagggaatgc atgcagtggg tcagtctgag atagtcattc
      361 ttctacagtg tttaccggat gaaaagtgtt tgccaaagga tatctttaat cactttgtgc
      421 agctttatcg ggatgctctg gcagggaatg tggtgagcaa cttgggacat tccttcttca
      481 gtcaaagttt ccttggcagt aaagaacatg gtggattctt atatgtgaca tctacctacc
      541 agtcactgca agacctagta ctcccaaccc caccttactt gtttgggatt cttatccaga
      601 aatgggaaac tccttgggct aaagtatttc ctatccgtct gatgttgaga cttggagctg
      661 aatatcgact ttatccatgc ccactattca gtgtcagatt tcggaagcca ttgtttggag
      721 agacggggca taccatcatg aatcttcttg cagacttcag aaattaccag tataccttgc
      781 cagtagttca aggtttggtg gttgatatgg aagttcggaa aactagcatc aaaattccca
      841 gcaacagata caatgagatg atgaaagcca tgaacaagtc caatgagcat gtcctggcag
      901 gaggtgcctg cttcaatgaa aaggcagact ctcatcttgt gtgtgtacag aatgatgatg
      961 gaaactatca gacccaggct atcagtattc acaatcagcc cagaaaagtg actggtgcca
     1021 gtttctttgt gttcagtggc gctctgaaat cctcttctgg ataccttgcc aagtccag
//
