LOCUS       sequence-8662-ex        3260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, 
            Greenblatt JF, Dilworth FJ, Rudnicki MA
  TITLE     Pax7 activates myogenic genes by recruitment of a histone 
            methyltransferase complex.
  JOURNAL   Nat Cell Biol. 2008 Jan . 10(1):77-84.
  PUBMED    18066051
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Jan . 10(1):77-84."
COMMENT     Sequence Label: pBRIT Pax7 N281
FEATURES             Location/Qualifiers
     source          1..3260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             331..747
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     677..699
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     715..731
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    758..791
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        798..816
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      871..880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      875..884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1726..1797
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    1815..1848
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1860..1880)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1865..2194
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2045..2180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2204..2803
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2204..2223)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2700..2720
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     gap             2804..2852
                     /estimated_length=49
ORIGIN
        1 tgcgtctgta ctagtagcta ctagctcgta tctggcggac ccgtggtgga actgacgagt
       61 tctgaacanc gggcgcaacc ntgggagacg tcccagggac tttggggccg tttttgtggc
      121 ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta
      181 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac
      241 cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg
      301 actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc cttaagtttg
      361 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
      421 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
      481 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
      541 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
      601 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
      661 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
      721 ccagccctca ctccttctct aggcgccggc cggatctata acttcgtata atgtatgcta
      781 tacgaagtta tggatctaat taaccctcac taaagggaac aaaagctgga gctccaccgc
      841 ggtggcggcc gctctagccc gggcggatcc gccaccatgg cggcgctgcc cggcgcggtc
      901 cccaggatga tgagacccgg cccggggcag aactacccgc gcaccggctt ccccctggaa
      961 gtgtccaccc ctcttggcca aggccgggtc aatcagcttg gtggggtctt catcaatggt
     1021 cgacccctgc cgaaccacat ccgtcacaag atagtggaaa tggcccacca tggcatccgg
     1081 ccctgcgtca tctcccgtca gctccgtgtt tctcatggtt gtgtctccaa gattctgtgc
     1141 cgatatcagg agactgggtc catccggccc ggggctatcg gaggcagcaa gcccagacag
     1201 gtggcgactc cggatgtgga gaaaaagatt gaggagtata agagagagaa ccccgggatg
     1261 ttcagctggg aaatccggga ccggctgctg aaggacggtc actgcgaccg aagcacggtg
     1321 ccctcagtga gttcgattag ccgagtgctc agaatcaagt tcgggaagaa agaggacgac
     1381 gaggaaggag acaagaaaga agaagatggc gagaagaaag ccaaacacag catcgatggc
     1441 atcctgggcg acaaagggaa ccgtctggat gagggctcag atgtggaatc agaacccgac
     1501 ctccccctga agcgcaagca gcgccgcagt cggaccacgt tcacagccga gcagctggag
     1561 gagctagaga aggcctttga gaggacccac tacccggaca tctacacccg ggaggagctg
     1621 gcacagagga ccaagctcac ggaggcacgc gtccaggtct ggttcagtaa ccggcgtgcc
     1681 cgctggcgca agcaggcagg agctaaccag ctggccgccc tcgaggacta caaggatgac
     1741 gatgacaagg attacaaaga cgacgatgat aaggactata aggatgatga cgacaaataa
     1801 tagtctagag atccataact tcgtataatg tatgctatac gaagttatct cgaccctgtg
     1861 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     1921 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     1981 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     2041 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     2101 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     2161 aggaggcttt tttggaggcc taggcttttg caaaaagctt accatgaccg agtacaagcc
     2221 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     2281 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     2341 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     2401 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     2461 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     2521 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     2581 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     2641 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     2701 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     2761 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgannnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcgataa aataaaagat tttatttagt
     2881 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     2941 acgccatttt gcaaggcatg gaaaaaatac ataactgaga atagagaagt tcagatcagg
     3001 tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     3061 tgcccggctc agggccaaga acagatggaa cagctgaata tgggccaaca gaatatctgt
     3121 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaaa tgcggtccag
     3181 ccctcagcag tttctagaga acatcagatg tttccagggt gcccaagact gaatgacctg
     3241 tgctatgact acatcagagc
//
