LOCUS       sequence-8664-ex        3554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, 
            Greenblatt JF, Dilworth FJ, Rudnicki MA
  TITLE     Pax7 activates myogenic genes by recruitment of a histone 
            methyltransferase complex.
  JOURNAL   Nat Cell Biol. 2008 Jan . 10(1):77-84.
  PUBMED    18066051
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Jan . 10(1):77-84."
COMMENT     Sequence Label: pBRIT Pax7 N33-170C
FEATURES             Location/Qualifiers
     source          1..3554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             331..747
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     677..699
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     715..731
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    758..791
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        798..816
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      871..880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      875..884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2020..2091
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    2109..2142
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2154..2174)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2159..2488
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2339..2474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2498..3097
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2498..2517)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2994..3014
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     gap             3098..3146
                     /estimated_length=49
ORIGIN
        1 tgcgtctgta ctagtagcta ctagctcgta tctggcggac ccgtggtgga actgacgagt
       61 tctgaacanc gggcgcaacc ntgggagacg tcccagggac tttggggccg tttttgtggc
      121 ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta
      181 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac
      241 cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg
      301 actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc cttaagtttg
      361 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
      421 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
      481 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
      541 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
      601 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
      661 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
      721 ccagccctca ctccttctct aggcgccggc cggatctata acttcgtata atgtatgcta
      781 tacgaagtta tggatctaat taaccctcac taaagggaac aaaagctgga gctccaccgc
      841 ggtggcggcc gctctagccc gggcggatcc gccaccatgg cggcgctgcc cggcgcggtc
      901 cccaggatga tgagacccgg cccggggcag aactacccgc gcaccggctt ccccctggaa
      961 gtgtccaccc ctcttgaatt catcaagttc gggaagaaag aggacgacga ggaaggagac
     1021 aagaaagaag aagatggcga gaagaaagcc aaacacagca tcgatggcat cctgggcgac
     1081 aaagggaacc gtctggatga gggctcagat gtggaatcag aacccgacct ccccctgaag
     1141 cgcaagcagc gccgcagtcg gaccacgttc acagccgagc agctggagga gctagagaag
     1201 gcctttgaga ggacccacta cccggacatc tacacccggg aggagctggc acagaggacc
     1261 aagctcacgg aggcacgcgt ccaggtctgg ttcagtaacc ggcgtgcccg ctggcgcaag
     1321 caggcaggag ctaaccagct ggccgccttc aaccaccttc tgccgggagg tttcccaccc
     1381 accggcatgc ccacgctgcc accctaccag ctgccggact ctacctaccc caccaccacc
     1441 atctcccaag atgggggcag cacagtacac aggccccagc cccttccgcc atcaaccatg
     1501 catcagggtg ggctggctgc ggccgctgca gcagcagaca ccagctctgc ctacggagcc
     1561 cgccacagct tctccagcta ctctgacagc ttcatgaacc ctggggctcc ctccaaccac
     1621 atgaaccctg tcagcaatgg cctgtctcct caggtcatga gcatccttag caacccgagt
     1681 gccgtgcctc cacagcccca ggccgacttc tccatctccc cgctgcatgg aggcctggac
     1741 tcggcttcct ccatctcagc cagctgcagc caacgggccg actccatcaa gccaggagac
     1801 agcttgccca cgtcccagtc ttactgccca cccacctaca gcaccactgg ctacagtgtg
     1861 gaccctgtgg ctggctacca gtacagccag tatggccaaa ctgctgttga ttacctggcc
     1921 aaaaacgtga gcctgtccac acagcgccgt atgaagcttg gggaacactc cgctgtgctg
     1981 ggacttcttc ctgtggaaac gggacaagcc tacctcgagg actacaagga tgacgatgac
     2041 aaggattaca aagacgacga tgataaggac tataaggatg atgacgacaa ataatagtct
     2101 agagatccat aacttcgtat aatgtatgct atacgaagtt atctcgaccc tgtggaatgt
     2161 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2221 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     2281 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     2341 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     2401 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     2461 cttttttgga ggcctaggct tttgcaaaaa gcttaccatg accgagtaca agcccacggt
     2521 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     2581 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     2641 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     2701 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     2761 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     2821 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     2881 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     2941 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     3001 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     3061 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgannn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnatcg ataaaataaa agattttatt tagtctccag
     3181 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     3241 ttttgcaagg catggaaaaa atacataact gagaatagag aagttcagat caggtcagga
     3301 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccg
     3361 gctcagggcc aagaacagat ggaacagctg aatatgggcc aacagaatat ctgtggtaag
     3421 cagttcctgc cccggctcag ggccaagaac agatggtccc caaatgcggt ccagccctca
     3481 gcagtttcta gagaacatca gatgtttcca gggtgcccaa gactgaatga cctgtgctat
     3541 gactacatca gagc
//
