LOCUS       sequence-86665-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derivative of pT7-agrBD-I with a point mutation in the agrD gene to 
            change AIP coding region from type-I to type-IV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Marchand N, Collins CH
  TITLE     Peptide-based communication system enables Escherichia coli to 
            Bacillus megaterium interspecies signaling.
  JOURNAL   Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975.
            Epub 2013 Jul 5.
  PUBMED    23775238
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24975";
            journalName: "Biotechnol Bioeng"; date: "2013-11"; volume: "110"; 
            issue: "11"; pages: "3003-12"
COMMENT     Sequence Label: pT7-agrBD-IV
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    64..88
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat
       61 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
      121 ggaattcagg agcccttcac catgaattat tttgataata aaattgacca gtttgccacg
      181 tatcttcaaa agagaaataa cttagatcat attcaatttt tgcaagtacg attagggatg
      241 caggtcttag ctaaaaatat aggtaaatta attgttatgt atactattgc ctatatttta
      301 aacatttttc tgtttacgtt aattacgaat ttaacatttt atttaataag aagacatgca
      361 catggtgcac atgcaccttc ttctttttgg tgttatgtag aaagtattat actatttata
      421 cttttacctt tagtaatagt aaattttcat attaactttt taattatgat tattttaaca
      481 gttatttctt taggtgtaat ctcagtatat gctcctgcag caactaaaaa gaagcccatt
      541 cctgtgcgac ttattaaacg aaaaaaatat tatgcgatta ttgttagttt aacccttttc
      601 attatcacac ttatcatcaa agagccattt gcccaattca ttcaattagg catcataata
      661 gaagctatta cattattacc tattttcttt attaaggagg acttaaaatg aatacattat
      721 ttaacttatt ttttgatttt attactggga ttttaaaaaa cattggtaac atcgcagctt
      781 atagtacttg ttacttcata atggatgaag ttgaagtacc aaaaga
//
