LOCUS       sequence-86747-e        1254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 558, Emission: 583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1254)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DsRed2-Mito-7
FEATURES             Location/Qualifiers
     source          1..1254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..212
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     163..183
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             241..315
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      343..352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             349..1026
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(529..549)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     941..964
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1147..1228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1184..1203)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
       61 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      121 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      181 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
      241 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
      301 gtgccgcgcg ccaagatcca ttcgttgggg gatccaccgg tcgccaccat ggcctcctcc
      361 gagaacgtca tcaccgagtt catgcgcttc aaggtgcgca tggagggcac cgtgaacggc
      421 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcca caacaccgtg
      481 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
      541 ttccagtacg gctccaaggt gtacgtgaag caccccgccg acatccccga ctacaagaag
      601 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      661 gcgaccgtga cccaggactc ctccctgcag gacggctgct tcatctacaa ggtgaagttc
      721 atcggcgtga acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag
      781 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagac ccacaaggcc
      841 ctgaagctga aggacggcgg ccactacctg gtggagttca agtccatcta catggccaag
      901 aagcccgtgc agctgcccgg ctactactac gtggacgcca agctggacat cacctcccac
      961 aacgaggact acaccatcgt ggagcagtac gagcgcaccg agggccgcca ccacctgttc
     1021 ctgtagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
     1081 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1141 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1201 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actc
//
