LOCUS       sequence-86782-e        1640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1640)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-PCNA-19-NLS-4
FEATURES             Location/Qualifiers
     source          1..1640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..58
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             77..757
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
ORIGIN
        1 tcagaatccg ctagccacca tggcttcgtg gggctccccg aagaagaagc gcaaagtact
       61 ggtaccggtc gccaccatga acagcctgat caaagaaaac atgcggatga aggtggtgct
      121 ggaaggcagc gtgaacggcc accagttcaa gtgcaccggc gagggcgagg gcaaccccta
      181 catgggcacc cagaccatgc ggatcaaagt gatcgagggc ggacctctgc ccttcgcctt
      241 cgacatcctg gccacatcct tcatgtacgg cagccggacc ttcatcaagt accccaaggg
      301 catccccgat ttcttcaagc agagcttccc cgagggcttc acctgggaga gagtgaccag
      361 atacgaggac ggcggcgtga tcaccgtgat gcaggacacc agcctggaag atggctgcct
      421 ggtgtaccat gcccaggtca ggggcgtgaa ttttcccagc aacggcgccg tgatgcagaa
      481 gaaaaccaag ggctgggagc ccaacaccga gatgatgtac cccgctgacg gcggactgag
      541 aggctacacc cacatggccc tgaaggtgga cggcggaggg cacctgagct gcagcttcgt
      601 gaccacctac cgatccaaga aaaccgtggg caacatcaag atgcccggca tccacgccgt
      661 ggaccaccgg ctggaaaggc tggaagagtc cgacaacgag atgttcgtgg tgcagcggga
      721 gcacgccgtg gccaagttcg ccggcctggg cggagggtcc ggagaagggc aagggcaagg
      781 gcaagggccg ggccgcggct acgcgtatcg atccatgttc gaggcgcgcc tggtccaggg
      841 ctccatcctc aagaaggtgt tggaggcact caaggacctc atcaacgagg cctgctggga
      901 tattagctcc agcggtgtaa acctgcagag catggactcg tcccacgtct ctttggtgca
      961 gctcaccctg cggtctgagg gcttcgacac ctaccgctgc gaccgcaacc tggccatggg
     1021 cgtgaacctc accagtatgt ccaaaatact aaaatgcgcc ggcaatgaag atatcattac
     1081 actaagggcc gaagataacg cggatacctt ggcgctagta tttgaagcac caaaccagga
     1141 gaaagtttca gactatgaaa tgaagttgat ggatttagat gttgaacaac ttggaattcc
     1201 agaacaggag tacagctgtg tagtaaagat gccttctggt gaatttgcac gtatatgccg
     1261 agatctcagc catattggag atgctgttgt aatttcctgt gcaaaagacg gagtgaaatt
     1321 ttctgcaagt ggagaacttg gaaatggaaa cattaaattg tcacagacaa gtaatgtcga
     1381 taaagaggag gaagctgtta ccatagagat gaatgaacca gttcaactaa cttttgcact
     1441 gaggtacctg aacttcttta caaaagccac tccactctct tcaacggtga cactcagtat
     1501 gtctgcagat gtaccccttg ttgtagagta taaaattgcg gatatgggac acttaaaata
     1561 ctacttggct cccaagatcg aggatgaaga aggatcttag ggatccaccg gatctagata
     1621 actgatcata aatcaggcca
//
