LOCUS       sequence-86800-e        2165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1405..1414
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1411..2121
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attcggcagc
       61 acttttcttt cattgggggg aaggcgtgag gaaagtacca aacagcagca gacttttaaa
      121 ctttaaacag acaggtctga gagcctgaac tctcattttt cctttgactt cagcctccaa
      181 ggagttccac caactttggc gcgccggctt cactttcatt aagtgaaaga gaggtgccca
      241 gacatgggtg actggagtgc cttggggaag cttctggaca aggtccaagc ctactccacc
      301 gctggaggga aggtgtggct gtcagtgctc ttcatattca gaatcctgct cctggggaca
      361 gctgttgagt cagcttgggg tgatgaacag tctgcctttc gctgtaacac tcaacaacct
      421 ggctgcgaaa acgtctgcta tgacaagtcc ttccccatct ctcacgtgcg cttctgggtc
      481 cttcagatca tattcgtgtc tgtgcccaca ctcctgtact tggcccatgt gttctatgtg
      541 atgaggaagg aagagaagct aaacaagaaa gaagaggagc tcaaagtggc ccagactgac
      601 ggggtcaacg tggagatgca cctgaagcag attgaaatca agaagttcaa gtacgggatt
      661 gaagagcacg gcaaggtgaa aatgaggggc ggcttgctga gaacctacat catcagcatc
      721 ctcttcaagt ctgtcttcga ggtggccttc ctgctcatcc agtggtacat ctatgggttc
      781 agcttgagcg cggtctacac ctgcaagaga gatccctgcc cccaccaggt agactgcttc
      841 ctctcacgtc ccacggagaa aaccatcttc atcatcttca tgctggtggt gtccttggtg
      901 tctctcgctt tgaacatcat tgagctcttc tacgtcttct tcaaaggcgt taaggatcgc
      961 gtgaagggaa gaagcgatcc ttaccacgcc accactggcc cactgagccc atcaaaagac
     1021 tgcggatctc caaaatacgc ctacttcaat ggctgctcct caccaacggc tccactctcg
     1081 cctatgtctc ctcctgggta caagctggtt actggtgaca gaaacaattc ctcgtgccgc
     1141 aattacaaca agcaagctag cgagcaaaac tgggcgaact acagcgcaga gcaaaatcgc
     1201 atggggcagg ccggaagcac catctccaac tcgcacgccc agccgttcga tttccccgac
     1261 gacaaccaga atgccaaaaa agttgctgct ggacatgaac tccagccatt agccatcgtg
     1321 gaccaacgac cttccagcag agccagcagc cgcgccagca gcaggcctcg gcctgatgac
     1381 ctggagattg cggatccacc ggtcgccacc atggtgtcta agggcgaaga gctgatcaag
     1441 gaaaatatgc gtatgaaggt ggtcatggaa ggttcggtca acggccacca attcaaatgc
     1501 acaggtgaag gagaaggcaa tccgtacatg ggaactcaaa ccatgaggat caaagtcatc
     1561 gagggaggac ccctgccatt tgcctttgac attcttgcca cgtcgttcat gtatggcagc
     1621 cgtactttta tcaagtaccc gaaaggcatt cctgatttct ttaaacagtc ctttcctgag
     1681 ggttttactt gggaaagagt tacgagatac gaagatggtg gagtcgtcac cgtcatgcag
     1741 gacaccagcc ttgaggatgg ctgtctcgtt taccacgtcc aagtcagagg ggtaaacttt
     1801 ccctccaatg gtcccgtgat gcagaagaag accaagggtt gggagcctaa tacagagatg
     1861 atgtatccag cagatggtgg tctgagggga tacactcata tggcactgaa agttgatggt
     1921 ggtggccatc tgtcttgctc tttcgtaaca acttacaggt caaaaaagac cgtcgggaac
     1981 atcaagatgc ccggtatcca tgccgttgat caccgcctgg aaaggttaga ggaaagtgac
     2041 aatgaaatgt tcgtagtaca acgcgaacac gcagttgcca agttcgccgg gcttggtggt
     2101 gggatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac
     2161 attgt
//
