LOCUS       sequence-86808-e        2078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(47..109,110..1267)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1322..1331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1328..2038
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc gccaccatgg cggccccggg
       61 cgcccggcgt ccgctgctcc tgttgctgct ggcaggcctt gcacacagcg ccccagcact
      121 gttcgaggtg aaagacaaca acggcacagc gtgtataatg gccagcttct ctgcctcctt
      181 tctgaccacc tatgaggctg gacatgtttc taaggtctcg aatatgaccc tgccagcctc
      241 tgcagaagtc ctgaagaata gcagctcttg tggtgaaaag aatgcttctg agcccaccct
      301 cgcaatcacc tttggagaag gatatttact gaaactcacc ttcacaaaaa acacaacacg
      361 ttacagtgtc cagcacatgt atttcacata taacctgtca gacacacaat tctttcccaa
      421 tgccagctcc aaagggcccg acactgtgga ttccacaact gacatcaagg cagacatcaa
      481 caaaacatac cgatgtgtca gcgacatcag ggtctacatg aagaatgtga ccattgtgct
      541 ctgggacgct actatccagg cctacctgcc gagtagcaac ttcagcaagg aagagacacg
      601 ctgcccacag gatcaacctt ccccaactac tgggccaccc agcccctcac caccacttgt
      661 gcccacaaac cccagtgtgt ccaagtacaa tgtgactggt gacaatggaa cctgcctgct
      721 ggcctctatg gcactgcaac tcaacatcac ctacatgaag aaggacaaca cgactgtgac
      781 cagagcattc aacatcaacc caagtgacaa atatagtggg acttgcggtg cccagttggt
      841 gaccctgaag gtggggaaca agagcagagt cctggagctg cagtttggga tgaatgccac
      901 ttctagcctg tttttcctgc aaggagttca gttgaacatg actcttcctg atgccataga
      961 gcccacgttc agcacctcca actattccct gaaagctctt caggccagtg tcggcaactc
     1021 atacaagtgc aacagtgagg agcacatctt tgtcagcaag gcgctcgccc tcaatgtctt
     1081 cagcgtgcaa gtccaggctt tcagggtaga aagtgacagg tttgggtctg tggaagagtg
     1141 tgtacaggac ggtaacaaca tgctgatccc cattgctgtg ggcggggccc tggcagggct
     1201 ggtcctcatc gtcctcatcg cctacctcat cggcaggaag aggagtcacg cgggctatca
     1261 gaccatctcg gaattcggct ccaccggctc caccggctcc accggcgcgg atccaccggt
     1321 cgccaccatg gtgtctaagg gcgaagagct gatcaaggaa aatatgcgta tgaaggtggt
     1381 catggaaggt tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag aaggcaatcc
     1441 gtacatggga actcaaacca tgaggatcaa agtcatcgag ggaggacccc tgccatttgc
     1501 ctttgacatt cttgccacgt cgttcatgta tggcagccgt acttttatca agtacccgaa
     1561 aggcattcct gatttcttta aacagtcctt tcctgagggt tttacttggg aaagagttac
     1621 gagatacgaa gatggtggag tcgtcaccgt catgcaggac accagccttg aggatggctg
     1681 tctcgtttac cacgtccaag tcagaggggt aaactttccc tccaatggtc ccgtgatgca
     1741 gaagaagacc aagggttggg agcctaatac agagatgatg tatccagcag atggtggtct
     1801 gaggggatac actcatatgg cactgaaagt tgatggtggt ggccatctgt cttgctcttt
     1861 cgtaacaact tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg gtatccatgc
     1921 cgttgatcac cgcctggaaa ggttagagga aagtgacaat gaaatgttcg tagtacaacg
     1981 cgaacacgca gttgccaagt tcgccgggct tggtggtggg atggacgagc tgtacaagta
     2041 agcggccgcg actctagatc ataatcagcc ataccaca
//
