LOCUS       sequence-86813-e        2313 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2313
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..743
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgtc taagggcgaa gagctgatca
       61 aggaaaatat gcgtatgaag gtggtcatgg aaggttcggt caacggccac caattcaaat
      121 gcacaggtga aggagaaggc aatccgtaca tgggaactca aaccatgagg atcaaagtca
      181 tcgagggagg acccctgcca tttgcctttg acattcttgc cacgtcgttc atgtatggca
      241 gccgtacttt tatcaagtac ccgaaaggca ttcctgattt ctttaaacag tcctttcctg
      301 agggttttac ttgggaaaga gttacgagat acgaagatgg tggagtcgtc accgtcatgc
      361 aggacaccag ccttgaggat ggctgtctcg tttaccacgt ccaagtcaga ggggtaaact
      421 ttccctccaa tggtcccgtg atgcagaaga agaccaaggg ttgggagcct aatacagaga
      481 tgatgtatcc agcagatggt ggtctgaggg gatacactca tatggcactg aaagttgatg
      541 gtggtggcca tctgtcttgc tctttcgtaa caacttacag gtcaaaaaag accgtcggga
      601 acatcaagat gcccggtatc catgccgttg atcaccgcct ggaaaggtta gaggaaagtg
      661 acaatgaaat gttcgtagta caacgcgaac acgcagttgc caagttcgcc gggcttggtg
      721 gtgggatgga cgagctgtac aagtccggaa gtgctgggag cgcaagcgga agcggaggct
      781 ccggttttaa ctacgaacgt ccaaaacact tcatccagtc ccaaaaccca tgtggctcca
      841 gattgcagcc tcctggacca gaaacctcca gcttctctag ccagaccaaa cagtcttcca
      901 ttatcatcca gccccgccag tgtacagagc aaagattttc tgcctcctca acactgagct
      961 ctcacatcac catgtcctcc tctgctttcc ctgcttctcc ccagcagcat gctggctcca
     1021 acccaggcca aagggttaca accacctata accagtcccc agccagcttc ctcagctcca
     1081 tattaccatc acagcctgat tacaatagca gtaaaatccc ttccgctatg gattccaact
     1141 atcaacagtc ctcagctggc caacctataa atgcaaagcc atcccaaact gcaaatgcta
     1201 agcccatacc aagaactcct gatcatgaaa tacaaggatc aaaagaagct ttgattcaag
     1261 atttggaaag aaagctgaaa tgcaaggaca cccttcttca taatggaaat caacgtctaa
     1321 catatgaaga gaagatggct cgcagattgc taggaccaca gaatgcagct gctgtgtttc
     1381 aagctcagga tgacagtggt gcacaagact cgcagcaaca caactcagaa catgcgcgac
     1441 tgcaagttcc tacatcacaa gtaagaagta gatcaacctc aaggggagat gtgaatgatc
     1501 aggatgcaat ccaggagaaa ttttacccac cacgtttcat tcaagtgcca gagaacatgt
     1561 cgattgatga aggaagattc tgcagaatgg acttcaaagt gagtggactg ccagctcctg
     1621 atgtgtcatg gtatctaaat ggaagaacag ttcaatcaga tgatttgcac aaaatgatag
     1681 tgtctgagaa gggtcttcat tcactcatct ttgaagtagt cagagcttca gatgcagggg
     1741 cttatgcatg tgttgccaag aatagagcag gagaagccac cttcactgtg cagctggatg
     1801 tccttgcaaa agaacataaa agagcaccaa tgtttatcta caaaccacag agcaaaaaag
     1861 ttttagaggg agattcagtg aaactagaat gccagatctc ggctatacct ccaccaaagc
     1921 ttttctggaa aagaaataat gaaatggtac aattcaacac tgaccgaata agcttatatc
     1981 aagataacac tggaagagtt actttactga taaaagatgt aaacaagaaa gatgctgggt
     2041 ggtatactgt gtcagcagtt aatgaagctg gagtgactac atgtaacaca agattagacg
     2101 ttacggcacg tccaaaccaa actcttccag ctcctaagca gttacgggtt cgaccaacat
     2161 tcagcaaata tttagcactt aatgggaaag gtttgaatgt aaaacaagct tttaacccag
     2221 aaggagaatt tcagcgtttg gcagctcaat ctggactcta tgaaagtgaa gaactttaag
     2281 gatccaccgg atctagataa ctgatcataa tca
//
