LOCUS       sequence-86821-e        2199 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2199
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             43..753
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 atgcttgtga cgtcagatcc gctagcgcta ccggtcgcca ccatggtgtc taagggcgaa
       61 gagctgatca aggaaaatat gcgtatgaag gtggtcatgg aaggttcggt caacggccac
      121 caattcaaat gcacaggtga aggagaaggc aatccgtaca tgggaactca aaccatgagg
      181 atcaaagtca tcgagggagg acccctgcca tttgcctttg acattcttgc cacgtcgttc
      241 atgtatggca gccgtacttt tatcaagtac ccgaaaggca ttcctgattt ctttaaacag
      301 tcctttcctg agggttttac ttgggaaaga gttacgagat acgaagatgg tggagtcgtc
      361 accgtcatgc aggacaccag ccttgaggat ggctgtctcg tttaccacgt ccaagtcaga
      421 ggggtaaact ttccctccaa tggtcccgtg atgcagaaga agaccaaggg ttgggagcct
      481 aatacagaga tgatgtatcc agcagatggt ggtctgaggg gatacactca tatggcactg
      541 aaagttgatg gtggtggcca tctgtcttgc tctttcgtaa caacttacag gtcaaaaaag
      601 accgtcggga acatcaagat gcccggtatc catgccgttg atcaccgcct ggaaaggtta
      661 gaggaaagtg acaatgaaat gttcgtagta caacgcgaac acgcagttgc caagttcgcc
      721 gggcttggtg gtgggatgga cgagctgtac aagtccggac tcagatctgg cagcggtgga
      781 ggcagcgcat ccggcggaag cggaagcgtg cgtgagtgca tctccatcca cgttggccag
      841 gctggtgtcc agattggcaa tgcctgctgg gagctctact gcctggaaca cggcatccag
      901 cccgatggcc agatgccaag tgacaagacc attgggggag gagatgactc cttcaacacc
      961 ttcttcagtg agacgggcgc tggcaagcac gtgccccggg ctgtgtttgt agacttggaa
     1021 cccacagtca ttgatgaagt tcgcactggc acctaccgcc agctcttcca ccctgagcag
     1081 ctcatcacag gcaaggaaga tgctgccaat aactatgccc gagggcacta caccattggc
     1141 aaggagatca ttgaccttgt gttggaccga attcgcaagc tggctgacca gtgcaccggt
     1201 cttcagggct tcttggtttt ccacagcttt ggtgggggaa ctggttctgg gttcacctcc
     1261 ctgctcatgg aacgtctctc agttgattat ggcaagaagt ccaagctgga gttctccatt
     1321 tacccagcac cccaggtttc cacagctgta gttgagccct acaactccat cctcaccacc
     1381 cacaccaccc tggagcactc tgattgtgcc ttcatggtag acaatgaggc catctatgac
     1441 atctgtcgta gaaacctcga tatcgagcgc ccaacctaca ctaaccttaa ccgccttatt
     1501 agccagattg tgtcctccat cactgcttcc ctgagatttg atggagccct gaatgttgac
     1561 ctgacagaat tccagaccaa cctggtgccc tacccccgca tccacttccc tctggccaca
     1621 tatgcccctg tcatctctgc tgagaaagcc taccatgaac agctttctgt agcagagatc
     1681 accaatgctt gctttgagcc agccaaccag atggtgaaat gtgaccctcg ccatggtaaa
     1741 tacatggctt gctgcctgtt gtaccgtggt gacgtggttc ccaaagatgt caatgctgcc
     1801 attgccacca tcaaaaccaa gcgcagcatc cagtttgtgg attggtgccc cactggcttc
     1861 aaggttggca tcaactacca gcctcccact gtggtgcctg gtggagacct ggccaaggta
     1921 cagagagctg tgtgcatgct gagcaacacc acagccattg ctgaggcctg ggctcgcctg
     1981 gaccacaagt ttgacctgat gtatgccaag cgtgcctttg ttcactggta cgtgggtgag
     2041 gggatggagg aaggcgagtt ttcagaggcc cgtgaagata tggctgccct tgagaaggat
     2101 tatgaggagg ttggtgtgga ttctgttgaa ggagagggtg aggaagaagg agaggaatac
     2161 taaggatcca ccggatctag ataactgatc ataatcagc
//
