LOCUS       sequence-86846-e        1689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 574, Emission: 
            596.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1689)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mStrawberry-EB3-7
FEATURES             Location/Qualifiers
     source          1..1689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             938..1648
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1083..1101)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1351..1370
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgactgcc accatggccg tcaatgtgta ctccacatct gtgaccagtg aaaatctgag
      121 tcgccatgat atgcttgcat gggtcaacga ctccctgcac ctcaactata ccaagataga
      181 acagctttgt tcaggggcag cctactgcca gttcatggac atgctcttcc ccggctgtgt
      241 gcacttgagg aaagtgaagt tccaggccaa actagagcat gaatacatcc acaacttcaa
      301 ggtgctgcaa gcagctttca agaagatggg tgttgacaaa atcattcctg tagagaaatt
      361 agtgaaagga aaattccaag ataattttga gtttattcag tggtttaaga aattctttga
      421 cgcaaactat gatggaaagg attacaaccc tctgctggcg cggcagggcc aggacgtagc
      481 gccacctcct aacccaggtg atcagatctt caacaaatcc aagaaactca ttggcacagc
      541 agttccacag aggacgtccc ccacaggccc aaaaaacatg cagacctctg gccggctgag
      601 caatgtggcc cccccctgca ttctccggaa gaatcctcca tcagcccgaa atggcggcca
      661 tgagactgat gcccaaattc ttgaactcaa ccaacagctg gtggacttga agctgacagt
      721 ggatgggctg gagaaggaac gtgacttcta cttcagcaaa cttcgtgaca tcgagctcat
      781 ctgccaggag catgaaagtg aaaacagccc tgttatctca ggcatcattg gcatcctcta
      841 tgccacagag gaaggattcg caccccctga ggacgatgag attgaagagc atcaacaaga
      901 agaccaggac gagtacctgg atccaccggt ggctaccatg gtgagcaagg gcgaggagaa
      961 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa
     1021 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     1081 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctaacccc
     1141 caacttcacc tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     1201 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     1261 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     1321 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     1381 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagat
     1441 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     1501 caagaagccc gtgcagctgc ccggcgccta catcgtcggc atcaagttgg acatcacctc
     1561 ccacaacgag gactacacca tcgtggaact gtacgaacgc gccgagggcc gccactccac
     1621 cggcggcatg gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata
     1681 ccacatgag
//
