LOCUS       sequence-86852-e        1929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1929)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Actin-12
FEATURES             Location/Qualifiers
     source          1..1929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..714
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     438..457
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     632..655
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ttgacgtcag atccgctagc gctaccggtg gctaccatgg tgagcaaggg cgaggaggtc
       61 atcaaggagt tcatgcgctt caaggagcac atggagggct ccgtgaacgg ccacgagttc
      121 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caggctgaag
      181 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gatcatgtac
      241 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
      301 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
      361 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaaggt gcgcggcacc
      421 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
      481 gagcggatgt accccgagga cggcgccctg aagggcgaga tgaagatgag gctgaggctg
      541 aaggacggcg gccactacga cgccgaggtc aagaccacct acatggccaa gaagcccgtg
      601 cagctgcccg gcgcctacaa gaccgacatc aagctggaca tcacctccca caacgaggac
      661 tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg cgcctccggt
      721 tctggtactg gttctactgg ttctcgagct atggatgatg atatcgccgc gctcgtcgtc
      781 gacaacggct ccggcatgtg caaggccggc ttcgcgggcg acgatgcccc ccgggccgtc
      841 ttcccctcca tcgtggggcg ccccaggcac cagggcgtga tggtgggcat gggtcagaag
      901 gattcctatg tgggcgacga ggcccagagc aagagaggca tcctcaccct gaagtacccc
      961 atcgagcacg gcatcgtcac caactgggac gacatggaga aaatctggca ccacaccttc
     1021 tacaatgagc tgcgtgtggc tcccgaggag caccccgtgc tgctgaccga ggcccccctg
     1081 aaccccaagg ccaaccgcga gaagatgacc cagatcatgt ttgagacctt caacacccca
     1141 gccatgtacg ttgctatcca ggctgtgcta tccctgtacg cctctggccg taccactggc
     1201 atcgtgatgg actccggtga cggggtcacc cacactgtgc ccatctacga ggggtatgcc
     1261 ctcccccatg ccatcctgcg tctggacctg gctggccggg acctgactga ctacctcatg
     1321 aagatcctca ccgagcgcgg ctacagcttc accaccacgg ccgagcggga aatcgtgcgt
     1381 gacattaagg agaagctgtg ctacgtcgcc ctggacttcg agcaagagat ggccacggct
     1441 gcttccagct cctccctgga gaagagctac gagctgcctg acggccaggt catcaccatt
     1501 ggcaatgagc ggttccgctg ccctgaggca ctcttccagc cttccttcct gggcatggag
     1561 tcctgtggca tccacgaaac taccttcaac tccatcatga agtgtgacgt ggacatccgc
     1621 aaagacctgt acgccaacac agtgctgtct ggcggcacca ccatgtaccc tggcattgcc
     1681 gacaggatgc agaaggagat cactgccctg gcacccagca caatgaagat caagatcatt
     1741 gctcctcctg agcgcaagta ctccgtgtgg atcggcggct ccatcctggc ctcgctgtcc
     1801 accttccagc agatgtggat cagcaagcag gagtatgacg agtccggccc ctccatcgtc
     1861 caccgcaaat gcttctaggg atccaccgga tctagataac tgatcataat cagccatacc
     1921 acattgaga
//
