LOCUS       sequence-86865-e        1708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1708)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Cx32-7
FEATURES             Location/Qualifiers
     source          1..1708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             989..1669
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     1390..1409
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1584..1607
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccctc
       61 tgggaaaggg cagcagcagc caggtgtggc agtgacaggg aggtgtgaat gaggcaggat
      121 gaactggaca ggtttgtaca ccttgctcag tggcgtgaac cggcattcta ctgccattgg
      181 ccgagtatgg ctctcggtca tcttcatctt cagaatcatg gtgctggtgg tggctgcaga
      241 gagtgtgtgg ggtgatgaga aatcttcctt catctgcaac acactccagc ctggctgcaa
      301 cagcgtttgc tatgaccaat tcttccccat ctcccatgtg cggctgtggt ccctgcagct
      361 catcctagtt tccaccccag ctctcctcgt ggccatgcac gtggctcacc agcaacacat
      421 agagaagaaa atgctacggc ttgagggcca tggggacccc ctacacctgg aggaggtgaa
      481 gaggcacaag gtccacatct cagggacact gtggtggacc tatgtcatca gcgtggtgtt
      541 ccggctgttg tttgaggccg tcttcatgta tgtcttttat ctgctctacc ctggctatgc
      601 catggtgcgg ctggtcaagt gcgacgtcta cccctgcccc aacacagtgg actgcttcgt
      661 gtcccgcccc accgagaaaa ccgtcttcac cgtcttcatg ctagctgcct ctggcatctg
      721 catcatcctc aatgtggccg aggtggtgta cctcatcatc cgggcctgtg cccgccgagc
      781 ccagcgccgc tccaatccac cttcccgcaa gggctcgggc ttcggccacc gcctctcacc
      841 tgaatacaag cagaatgaga tcaacaagct gctgagtgag caggatggct ccctgaaaga
      901 catactgcgc cgcagccctg gcaccggggc tgggctggct gaaaagagcg accgctgctc
      961 ggcctgcgcg gatccaccgg tggctaccat ggtgagcaag ggcgaggagg tcatcaagga
     1021 gttcatgcgc ttcaaggagc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     1081 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaggctga aggtgaccaa
     1141 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagatcatgt acggctccaa
     1201 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     1261 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     1321 ctcctccctg caggacggcg agttcatcta caaggtgaag gtgcgcggca ccaacttccc
     1381 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     1441 gtaccccgag gacggcgccc tgaagggcga gatgaagatg aggctgaggc tgaaggacgg
     1501 cggccactac gacgccgagg tcaagaccac ctacatggcc aagaagcccg tgcagctgcc
     1561 cggcgcctac aagaccgaca tcaagctgga catcacctcc cacaacgagg actacaccat
     1621 cgtggaacag tacgagcgcg ccgagggccg ccactccacc ggcgcctaag cggccgcgac
     1681 tctagatcat aatcagccat accacatt
//
