LOCUS       sequence-86869-e        1657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 590, Emission: 
            649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1657)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-EB3-7
FEATURES             Location/Qualifiers
     source          1..1657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             938..1618
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     1339..1358
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1533..1556
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgactgcc accatggccg tcaatgtgta ctccacatct gtgaccagtg aaaatctgag
      121 tcgccatgat atgcttgcat gggtcaacga ctccctgcac ctcaactata ccaagataga
      181 acagctttgt tcaggggcag cctactgcca gttcatggac atgctcttcc ccggctgtgt
      241 gcacttgagg aaagtgaagt tccaggccaa actagagcat gaatacatcc acaacttcaa
      301 ggtgctgcaa gcagctttca agaagatggg tgttgacaaa atcattcctg tagagaaatt
      361 agtgaaagga aaattccaag ataattttga gtttattcag tggtttaaga aattctttga
      421 cgcaaactat gatggaaagg attacaaccc tctgctggcg cggcagggcc aggacgtagc
      481 gccacctcct aacccaggtg atcagatctt caacaaatcc aagaaactca ttggcacagc
      541 agttccacag aggacgtccc ccacaggccc aaaaaacatg cagacctctg gccggctgag
      601 caatgtggcc cccccctgca ttctccggaa gaatcctcca tcagcccgaa atggcggcca
      661 tgagactgat gcccaaattc ttgaactcaa ccaacagctg gtggacttga agctgacagt
      721 ggatgggctg gagaaggaac gtgacttcta cttcagcaaa cttcgtgaca tcgagctcat
      781 ctgccaggag catgaaagtg aaaacagccc tgttatctca ggcatcattg gcatcctcta
      841 tgccacagag gaaggattcg caccccctga ggacgatgag attgaagagc atcaacaaga
      901 agaccaggac gagtacctgg atccaccggt ggctaccatg gtgagcaagg gcgaggaggt
      961 catcaaggag ttcatgcgct tcaaggagca catggagggc tccgtgaacg gccacgagtt
     1021 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaggctgaa
     1081 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agatcatgta
     1141 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     1201 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     1261 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagg tgcgcggcac
     1321 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     1381 cgagcggatg taccccgagg acggcgccct gaagggcgag atgaagatga ggctgaggct
     1441 gaaggacggc ggccactacg acgccgaggt caagaccacc tacatggcca agaagcccgt
     1501 gcagctgccc ggcgcctaca agaccgacat caagctggac atcacctccc acaacgagga
     1561 ctacaccatc gtggaacagt acgagcgcgc cgagggccgc cactccaccg gcgcctaagc
     1621 ggccgcgact ctagatcata atcagccata ccacatt
//
